| 偶联酶法检验L-天冬酰胺酶的动力学分析 |
| |
| 引用本文： | 张学忠. 偶联酶法检验L-天冬酰胺酶的动力学分析[J]. 吉林大学学报(理学版), 1989, 0(3) |
| |
| 作者姓名： | 张学忠 |
| |
| 作者单位： | 吉林大学酶工程实验室 |
| |
| 摘 要： | 根据McClure偶联酶反应理论,通过实验对偶联酶法检验L-天冬酰胺酶活力的反应条件进行了动力学分析,确定了合理的附加酶用量,建立了偶联酶法检验此酶活力程序。用该程序测定的底物K_m值和产物的抑制常数K_1值与文献值相符。
|
| 关 键 词： | L-天冬酰胺酶检验 偶联酶法 动力学 |
A Kinetic Analysis of Couple Enzyme Assay for L-Asparaginase |
| |
| Zhang Xuezhong. A Kinetic Analysis of Couple Enzyme Assay for L-Asparaginase[J]. Journal of Jilin University: Sci Ed, 1989, 0(3) |
| |
| Authors: | Zhang Xuezhong |
| |
| Abstract: | A kinetic analysis of couple enzyme assay for L-asparaginase was made on the basis of Mc-Clure's theory. A correct amount of auxiliary enzyme (Glutamate Dehydrogenase) was calculated. A procedure of continuous assay for L-asparaginase with GDH as a couple enzyme was designed. As an application of the couple enzyme assay, the determination of apparent K_mof L-asparagine and K_1of L-aspartic acid was carried out. |
| |
| Keywords: | enzyme assay couple enzyme assay kinetics. |
| 本文献已被 CNKI 等数据库收录！ |
