| 时间分辨荧光免疫分析法测定牛血清白蛋白 |
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| 引用本文： | 慈云祥 张乃昌. 时间分辨荧光免疫分析法测定牛血清白蛋白[J]. 北京大学学报(自然科学版), 1994, 30(4): 413-420 |
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| 作者姓名： | 慈云祥 张乃昌 |
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| 作者单位： | 北京大学化学系 |
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| 摘 要： | 用Ｅｕ－ＴＴＡ络合物标记了牛清白蛋白和抗牛血清白蛋白的兔血清。分另用竞争法和夹心法测定了牛血清白蛋白。研究结果表明ＴＴＡ－ＳＯ２Ｃｌ是一种标记效率较高的的双功能络合剂,可获得较高的结合比（ＴＴＡ：ＢＳＡ＝３５：１;ＴＴＡ：ａｎｔｉ－ＢＳＡ＝１００：１）。用时间分辨荧光免疫分析法测定了牛血清白蛋白。竞争法和夹心法的检测下限分别为１５０ｎｇ／ｍＬ和５０ｎｇ／ｍＬ。
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| 关 键 词： | 免疫分析法 牛 血清白蛋白 络合物 |
Time-Resolved Fluoroimmunoassay of Bovine serum Albumin with Eu-TTA as Label |
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| CI Yunxiang CHANG Wenbao ZHOU Bo. Time-Resolved Fluoroimmunoassay of Bovine serum Albumin with Eu-TTA as Label[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1994, 30(4): 413-420 |
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| Authors: | CI Yunxiang CHANG Wenbao ZHOU Bo |
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| Abstract: | Bovine serum albumin(BSA)and anti-BSA antiserum were labelled by Eu-TTA chelate. BSA was quantified by competitive assay and "sandwich"assay,respectively. The results showed that the home svnthesized TTA-SO_2Cl was a suc cessful bifunctional ligand,high conjugation ratios(TTA:BSA = 35:1,TTA:anti-BSA=100:1)were obtained.Time-resolved fluoroimmuno assay could detect BSA sensitively,the limits of detection with competitive assay and " sandwich"assay were 150 ng/mL and 50 ng/mL,respectively. |
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| Keywords: | time-resolved fluoroimmunoassay bovine serun1 albumin Eu-TTA complex |
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