| 铁皮石斛不同繁殖代数遗传稳定性RAPD的研究 |
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| 引用本文： | 刘石泉,李小军,周根余. 铁皮石斛不同繁殖代数遗传稳定性RAPD的研究[J]. 江南大学学报(自然科学版), 2005, 4(5): 518-521 |
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| 作者姓名： | 刘石泉 李小军 周根余 |
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| 作者单位： | 1. 湖南城市学院,化学与环境工程系,湖南益阳,413049
2. 上海师范大学,生命与环境科学学院生物系遗传学实验室,上海,200234 |
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| 基金项目： | 上海市高等学校科学技术发展基金项目（03DZ02）资助课题. |
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| 摘 要： | 研究铁皮石斛单蒴果种子内不同无性繁殖代数的遗传稳定性.利用从100个随机引物筛选出23个稳定性较好的10碱基引物,对来自单蒴果的7代无性繁殖种苗进行RAPD检测.发现7代无性繁殖的组培苗内代代之间遗传距离很小,但代数相差越大其遗传距离就越大,在0～0.043 5范围内.其中在1～3代未探查到变异,但从第4代始,有部分引物RAPD带型发生变化,但变化甚微.23个稳定引物只探查到了7个变异位点.铁皮石斛组培过程中种内存在一定的变异,但变异甚微,所以建立铁皮石斛稳定的无性快繁体系是切实可行的.
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| 关 键 词： | 铁皮石斛 随机扩增多态性DNA 蒴果 繁殖代数 遗传稳定性 |
| 文章编号： | 1671-7147（2005）05-0518-04 |
| 收稿时间： | 2004-11-25 |
| 修稿时间： | 2004-11-252005-01-20 |
Study on Hereditary Stability for Dendrobium o f ficinale et Migo of Different Descends |
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| LIU Shi-quan,LI Xiao-jun,ZHOU Gen-yu. Study on Hereditary Stability for Dendrobium o f ficinale et Migo of Different Descends[J]. Journal of Southern Yangtze University:Natural Science Edition, 2005, 4(5): 518-521 |
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| Authors: | LIU Shi-quan LI Xiao-jun ZHOU Gen-yu |
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| Affiliation: | 1. Department of Chemistry and Environment Engineering, Hunan City University, Hunan Yiyang, 413049, China;2. College of Life and Environment Science, Shanghai Teachers University, Shanghai, 200234, China |
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| Abstract: | To research the hereditary stability of Dendrobium officinale et Migo which come from different descends.Using 23 primers of arbitrary decamer oligonucleotide sequences were screened from 100 primers for DNA amplification,random amplified polymorphic DNA(RAPD) were used.The genetic distance of adjacent generations are very slight,varying from 0 to 0.0435,the more far,the more big.The variation of these descends were detected since the 4th descends.Only 7 variation sites were detected in 23 primers.The variation of the same pad is smaller.To set up the rapid propagation system of Dendrobium officinale et Migo is feasible. |
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| Keywords: | random amplified polymorphic DNA(RAPD) Dendrobium officinale et Migo silique different descends hereditary stability |
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