组蛋白甲基化修饰在小鼠早期胚胎发育中的建立与调控

 组蛋白修饰作为重要的表观遗传修饰，在调控胚胎基因表达、胚胎细胞的命运决定及胚胎基因组的稳定性等方面均起了很重要的作用。微量测序技术的发展使从全基因组水平上检测植入前胚胎的组蛋白修饰成为可能。综述了近年来利用该技术对小鼠早期胚胎发育过程中的组蛋白甲基化修饰研究的最新进展，总结了在胚胎基因激活及第一次细胞分化过程中组蛋白H3K4me3和H3K27me3修饰不同的建立和动态变化趋势，这些研究为探索胚胎发育和细胞分化的表观调控机制奠定了基础。

Histone modification dynamics during mouse pre-implantation development

Histone methylation, as an important epigenetic modification, plays a critical role in gene expression regulation as well as in the first cell fate determination during pre-implantation development. Low input new-generation sequencing provides us a reliable method to assess genome-wide histone modification reprogramming with high resolution using a very small number of cells. In this article we review the current advances of histone modification dynamics during pre-implantation development obtained through low-input sequencing technologies. Particularly, we highlight the establishment and dynamic of histone H3K4me3 and H3K27me3 modifications during zygotic genome activation and the first lineage differentiation. These advances have shed light on the mechanisms of epigenetic regulation network controlling early development and differentiation.