分支DNA探针法检测21例丙型肝炎血清病毒RNA

目前主要依赖检测丙型肝炎抗体来确定对丙型肝炎病毒(Hepatitis C virus,HCV)感染的诊断,但它不能反应机体是否有活动的病毒血症。分支DNA探针法应用合成的DNA分子与靶HCV—RNA特异性的杂交,形成RNA—DNA杂交体,用dioxetane作为底物与化学发光物结合,通过测定其发光强度可直接检测血清HCV—RNA的含量。本文测定21例慢性活动性丙型肝炎血清。HCV—RNA最低值为0.66Meg/ml;最高值为58Meg/ml,21例中86％的数值分布在0.66Meg/m-12Meg/ml的范围内。此方法cutoff值为0.5Meg//ml。我们所测定的21例均高于cutoff值。此方法操作简便,特异性强。为临床丙型肝炎治疗的监测及疗效的判断提供了重要的依据。

The Detection of Serum HCV - RNA in 21 Cases of Chronic Active Hepatitis C with Branched DNA Probe Assay

Currently, hepatitis C virus (HCV) infection is chiefly diagnosed by detecting circulating HCV antibodies, the presence of which cannot, however, indicate the exact existence of active viremia. In branched DNA probe assay, synthetic DNA molecules are used to combine with HCV- RNA to form specifically a double - stranded DNA- RNA hybridization. The synthetic DNA molecules possess multiple DNA branches for signal amplification of nucleic acid targets, and combined with chemiluminescence detection using dioxetane dervative as a substrate, HCV- RNA was assayed for 21 patients with chronic active hepatitis C, with the lowest value of 0.66 Meq/ml and the highest value of 58 Meq/ml. The values of HCV - RNA in all the 21 cases were beyond the cut off value(0. 5Meq/ml). This method is simple in manipulation with high specificity, and offers an important basis for monitoring the therapeutic course of and evaluating the therapeutic effects on clinical hepatitis c.