Preparation of a specific ligand for the purification of arylsulfatases by affinity chromatography |
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| Authors: | C Derappe G Dubois |
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| Institution: | (1) I.N.S.E.R.M. U. 98, 17, rue du Fer à Moulin, F-75005 Paris, (France);(2) Present address: Laboratoire de Neurochimie C.N.R.S.E.R.A. 421, Hôpital de la Salpêtrière, 47, Bld. de l'Hôpital, F-75634 Paris Cédex 13, (France) |
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| Abstract: | Summary The reduction ofp-nitrocatechol sulfate, the artificial substrate of arylsulfatases followed by a condensation of a succinic anhydride, gives a ligand for the purification of the enzyme by affinity chromatography. |
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