| 墨天牛属三个近缘种的RAPD分析 |
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| 引用本文： | 安榆林,刁彩华.墨天牛属三个近缘种的RAPD分析[J].南京林业大学学报(自然科学版),1998,22(4):35-38. |
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| 作者姓名： | 安榆林 刁彩华 |
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| 作者单位： | 中华人民共和国南京动植物检疫局 |
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| 基金项目： | 国家动植物检疫局“九·五”重点课题 |
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| 摘 要： | 应用ＲＡＰＤ（随机扩增的多态性ＤＮＡ）技术，分析了墨天牛属三个近缘种－松墨天牛、云杉大墨天牛和云杉小墨天牛的ＤＮＡ多态性。通过采用多种引物对同种天牛幼虫的浸渍标本和新鲜标本的ＤＮＡ多态性研究，发现浸渍标本与新鲜标本ＤＮＡ扩增后的电泳指纹图谱完全一致；同种害虫不同虫态间的ＤＮＡ指纹图谱完全一致；三种随机引物可明显鉴别三种天牛幼虫。这为天牛近缘种的鉴定，尤其是天牛幼虫的鉴定提供了一种新方法。
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| 关 键 词： | 墨天牛属 近缘种 幼虫 RAPD 鉴定 |
RAPD ASSESSMENT OF THREE SIBLING SPECIES OF MONOCHAMUS GUER ( COLEOPTERA: CERAMBYCIDAE ) |
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| An Yulin,Diao Caihua,Zhu Hongbing,Jiao Guoyao.RAPD ASSESSMENT OF THREE SIBLING SPECIES OF MONOCHAMUS GUER ( COLEOPTERA: CERAMBYCIDAE )[J].Journal of Nanjing Forestry University(Natural Sciences ),1998,22(4):35-38. |
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| Authors: | An Yulin Diao Caihua Zhu Hongbing Jiao Guoyao |
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| Institution: | Nanjing Animal and Plant Quarantine Service Nanjing 210009 |
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| Abstract: | Three sibling species, Monochamus alternatus Hope, M. urussovi Fischer and M. sutor Linnaeus were analysed by using randomly amplified polymorphic DNA (RAPD) method. The result indicated that three primers were evident to separate those three sibling species. Whereas those in different stages of same species were almost of no difference. DNA pured from soaked and live individuals of cerambycid larvae for RAPD reactions using several primers resulted in identical RAPD fingerprints. Consequently, it is an available method newly provided by the authors for identification of sibling cerambycid species, especially for cerambycid larvae. |
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| Keywords: | Monochamus Guer Sibling Species Larvae RAPD Identification |
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