| Cloning, sequencing and expression of the coat protein gene of Cocksfoot mottle virus |
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| 作者姓名： | DONG Zhi GUO Xiaoyu ZHANG Hailong and ZHANG Feiyun |
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| 作者单位： | College of Life Science，Capital Normal University，Beijing 100037，China; College of Resources Environment and Tourism，Capital Normal University，Beijing 100037，China; Beijing Key Laboratory for Resources Environment and Geography Information System，Beijing 100037，China |
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| 基金项目： | Supported by National Natural Science Foundation of China (Grant No. 30571010)，Key Program of Scientific and Technological Research of Ministry of Education (No. 204006) |
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| 摘 要： | The coat protein (CP) gene of Cocksfoot mottle virus (CfMV) was amplified by RT-PCR and inserted into expression vector pGEX-4T-1, and the resulting plasmid was designated as pGEXCfMV-JANCP. The fusion protein GST-CP was expressed in BL21 (DE3) pLysS after IPTG induction. The results of SDS-PAGE and Western blot analysis showed that the CfMV-CP gene was efficiently expressed in E. coli BL21 (DE3) pLysS through IPTG induction and the 56.0 kD protein was obtained.
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Cloning, sequencing and expression of the coat protein gene of Cocksfoot mottle virus |
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| DONG Zhi,GUO Xiaoyu,ZHANG Hailong and ZHANG Feiyun.Cloning, sequencing and expression of the coat protein gene of Cocksfoot mottle virus[J].Progress in Natural Science,2007,17(2):222-225. |
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| Authors: | DONG Zhi GUO Xiaoyu ZHANG Hailong |
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| Abstract: | The coat protein (CP) gene of Cocksfoot mottle virus (CfMV) was amplified by RT-PCR and inserted into expression vector pGEX-4T-1, and the resulting plasmid was designated as pGEXCfMV-JANCP. The fusion protein GST-CP was expressed in BL21 (DE3) pLysS after IPTG induction. The results of SDS-PAGE and Western blot analysis showed that the CfMV-CP gene was efficiently expressed in E. coli BL21 (DE3) pLysS through IPTG induction and the 56.0 kD protein was obtained. |
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| Keywords: | Cocksfoot mottle virus sequence analysis prokaryotic expression |
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