光敏核不育杂交稻种子纯度的研究

本文利用等电点聚焦电泳测定酯酶同工酶以鉴定光敏核不育种子纯度;胚芽的酯酶同工酶谱能真实地显现该品种的特征,用作种子的鉴定材料为好;Ks-9/T370为互补型同工酶谱,而W6154/特青为杂种型酶谱;严重劣变的种子,在等电点聚焦电泳下,仍能保持该品种特征的酯酶同工酶谱。

Identification of HPGMR Cultivars by Electrophoretic Patterns of Esterase Isozymes

The esterase isozymes of seeds were determined in the early germination. The number of isozymes and the content of esterase in plumule were the largest and represented the characteristics of cultivars If the isozymes of radicle were mixed with that of plumule, the large bands of isozymes covered the small bands of isoyzmes and failed to show the whole pattern. It was suggested that use of plumule as material for the electrophoresis to identify the cultivar will be more accurate. The isozyme pattern of Ks-9/T370 (HPGMR) was complement- ary type correspondingly to its parents Ks-9 and T370 cultivars. Whereas, the isozyme pattern of W6154/Te-qing(HPGMR) had only two isozymes came from the parents and the others did not originated from the parents, so that the isozyme pattern was hybridization type. Therefore, genes of the parents of W6154/Te-qing had greater difference, whereas those of Ks-9/T370 had less difference. When seeds of HPGMR were stored in 75%RH for four months, viability and vigour decreased to a very low level. Though seeds deteriorated seriously, the isozyme patterns of esterase can be maintained. Owing to the stability of the isozymes of esterase during ageing, the isozymes pattern can be used as a good index in identification of cultivars or strains.