铜绿微囊藻附生细菌多样性及其溶磷活性研究

为了解铜绿微囊藻附生细菌的多样性及其溶磷活性,采用平板稀释涂布法分离细菌,并利用16S rRNA基因序列分析确定其分类学地位,用钼锑抗比色法检测其溶磷活性,用苯磷酸二钠法检测其磷酸酶活性.结果表明:分离的细菌分属于4个门,即放线菌门、变形菌门、拟杆菌门和异常球菌-栖热菌门; 其中83%以上的细菌至少对卵磷脂、植酸钙和磷酸钙中的1种具有溶磷活性,94%以上的细菌表现出1～3种磷酸酶活性,且导致磷培养基的pH值下降.铜绿微囊藻附生细菌中存在丰富的溶磷微生物,这些微生物对藻类获取足够的可溶性磷元素可能具有重要的

The Diversity and Phosphate Solubilization Activity of Microcystis aeruginosa-Associated Bacteria

In order to understand the diversity and phosphate solubilization activity of Microcystis aeruginosa-associated bacteria,dilution plating procedure is used to isolate the bacteria,and the 16S rRNA gene sequences are used to determine the taxonomic status of the bacteria,the molybdenum antimony resistance colorimetric and disodium phenyl phosphate methods are used to analyze solubilizing-phosphate activities and phosphatase activities of the bacteria,respectively.The results show that the isolates belong to four phylums,i.e.Actinobacteria,Proteobacteria,Bacteroidetes and Deinococcus-Thermus,respectively.And more than 83% of these bacteria exhibit solubilizing-phoshate activities at least on one of the three phosphorus sources such as lecithin,phytin and tricalcium phosphate,and more than 94% of these bacteria exhibit 1～3 kinds of phosphatases,and result in decline of the pH value of phosphorus media.There are abundant solubilizing-phosphate bacteria in the Microcystis aeruginosa-associated bacteria,and these microorganisms probably play an important role for the algae to obtain enough soluble phosphorus.