Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale |
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Authors: | Herring Christopher D Raghunathan Anu Honisch Christiane Patel Trina Applebee M Kenyon Joyce Andrew R Albert Thomas J Blattner Frederick R van den Boom Dirk Cantor Charles R Palsson Bernhard Ø |
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Affiliation: | Department of Bioengineering, University of California, San Diego, California 92093, USA. |
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Abstract: | We applied whole-genome resequencing of Escherichia coli to monitor the acquisition and fixation of mutations that conveyed a selective growth advantage during adaptation to a glycerol-based growth medium. We identified 13 different de novo mutations in five different E. coli strains and monitored their fixation over a 44-d period of adaptation. We obtained proof that the observed spontaneous mutations were responsible for improved fitness by creating single, double and triple site-directed mutants that had growth rates matching those of the evolved strains. The success of this new genome-scale approach indicates that real-time evolution studies will now be practical in a wide variety of contexts. |
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