Fibrillarin redistributes to the spindle poles and partially colocalizes with NuMA during mitosis |
| |
Authors: | Yan Guo Chaoliang Wei Jinsong Hu Mingxiao Ding Jianguo Chen |
| |
Institution: | (1) Department of Cell Biology and Genetics, College of Life Sciences, Peking University, 100871 Beijing, China |
| |
Abstract: | Fibrillarin, a major protein in the nucleolus, is known to redistribute during mitosis from the nucleolus to the cytosol,
and is related to the dynamics of post-mitotic reassembly of the nucleolus. To better understand the dynamic behavior and
the relationship with other cytoplasmic structures, we have now expressed fibrillarin-pDsRed1 fusion protein in HeLa cells.
The results showed that a part of fibrillarin was associated with mitotic spindle poles in the mitotic cells. Nocodazole-induced
microtubule depolymerization resulted in fibrillarin redistribution throughout the cytoplasm, and removal of nocodazole resulted
in relocalization of fibrillarin at the polar region during the mitotic spindles reassembly. In a mitotic cell free system,
fibrillarin was found in the center of taxol-induced microtubule asters. Moreover, fibrillarin was found to colocalize with
the nuclear mitotic apparatus protein (NuMA) at the poles of mitotic cells. Therefore, it is postulated that the polar redistribution
of fibrillarin is mediated by microtubules. |
| |
Keywords: | fibrillarin mitotic spindle poles centrosome NuMA |
本文献已被 万方数据 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |