不同民族大学生乙肝病毒基因型分布状况调查

为了解不同民族大学生乙型肝炎病毒（hepatitis B virus,HBV）基因型和亚型分布状况,采用限制性片段长度多态性聚合酶链反应（PCR-RFLP）技术,对中南民族大学93例来自15个民族乙肝大三阳学生血清HBV进行基因分型.结果表明：患者中HBV基因B型占61.3%（57/93）,C型占25.8%（24/93）,D型占4.3%（4/93）.说明各民族HBV基因型分布中,B型占绝对优势,C型次之,D型最少（P〈0.01）.且不同民族不影响HBV基因型的分布（P〉0.05）,C型和D型分布基本符合我国HBV基因型地理区域分布规律.

Study on the Distribution of HBV Genotype among Undergraduates from Different Nationalities

To investigate the distribution of Hepatitis B virus genotype and subgenotype among undergraduates from various nationalities,93 HBV-DNA positive serum samples from 15 nationalities in South-Central University for Nationalities were analyzed by PCR-RFLP.The results indicated that HBV genotype B accounted for 61.3%(57/93),genotype C accounted for 25.8%(24/93) and genotype D accounted for 4.3%(4/93).This demonstrated that genotype B is dominant in all the nationalities,genotype C ranks secondly and least genotype D(P<0.01).Ethnic differences have no effect on the classification of HBV genotype(P>0.05).Moreover,distribution of genotype C and D basically coincides with the geographic distribution of HBV in China.