| 湘西辣椒花药离体培养及其再生植株染色体数目的变异 |
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| 引用本文： | 吴光辉,黄丽芳,蒋建雄.湘西辣椒花药离体培养及其再生植株染色体数目的变异[J].吉首大学学报(自然科学版),2012,33(1):81-84. |
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| 作者姓名： | 吴光辉 黄丽芳 蒋建雄 |
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| 作者单位： | （1．湘西自治州农业科学研究所，湖南 吉首 416000；2．湖南农业大学生物科学技术学院，湖南 长沙 410128） |
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| 摘 要： | 利用辣椒花药诱导单倍体研究表明：NTH，MS培养基均能诱导产生愈伤组织和胚状体，但NTH培养基比MS培养基培养效果好，以NTH+6-BA 0.2mg/L+NAA 0.5mg/L+KT 1.2mg/L+AgNO3 5.0 mg/L为愈伤组织和胚状体诱导的适宜培养基。通过花药培养获得的辣椒植株中，具有丰富的倍性变异，经染色体计数鉴定，单倍体( n=12 )约14%，二倍体(2n=24 )约46%，四倍体(4n=48 ) 约4%，各种混倍体约36%.
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| 关 键 词： | 辣椒 花药培养 染色体数目变异 |
Anther in Vitro Culture of Capsicum Annuum and Its Chromosome Number Variation |
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| WU Guang-Hui,HUANG Li-Fang,JIANG Jian-Xiong.Anther in Vitro Culture of Capsicum Annuum and Its Chromosome Number Variation[J].Journal of Jishou University(Natural Science Edition),2012,33(1):81-84. |
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| Authors: | WU Guang-Hui HUANG Li-Fang JIANG Jian-Xiong |
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| Institution: | (1.Agricultural Institute of Xiangxi Autonomous Prefecture,Jishou 416000,China；2.College of Bioscience & Biotechnology,Key Laboratory of Cell Engineering,Hunan Agricultural University,Changsha 410128，China) |
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| Abstract: | The haploid induction from Capsicum annuum through anther culturing was studied.The results showed that both of the calli and embryos were induced on NTH as well as MS basal medium but the medium of NTH + 6-BA 0.2 mg/L + NAA 0.5 mg/L + KT 1.2mg/L + AgNO3 5.0 mg/L was the optimal medium for inducing calli and embryos.Several kinds of plants in different ploidy levels were obtained through anther culture,14% of which were the haploids,46% the diploids,4% the tetraploids,and 36% the mixoploids. |
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| Keywords: | capsicum annuum anther culture chromosome number variation |
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