金属离子在煤生物转化过程中的激活作用研究

研究了5种外源性金属离子对液相和固相培养的煤生物转化率的影响。在5种金属离子对酶活性的影响上,真菌和细菌表现出了不同的金属离子敏感性,大多数金属离子对真菌有激活作用,其中Mn2+在本实验条件中下,对真菌A有最好的激活作用,生物转化率提高了120%;Zn2+将真菌B的生物转化率从8%提高到29%,提高了263%;Fe3+对细菌的溶煤作用有较好的促进效果,生物转化率提高了184%.研究结果表明,细菌转化液总的蛋白含量与金属离子对煤转化率的影响趋势是正相关的。

Activation effect of metalions for bioconversion of coal

The aim of this study is to explore the influence of five metal ions on the coal biological trans- formation using liquid or solid medium. Fungus and bacteria exhibited different metal ions sensitivities on the influence to enzyme activities by five metal ions, most metal ions showed activation effect on fungus, and under the experimental conditions in this study, Mn^2＋ conduct the best activation effect on fungus A with a 120% elevation of biotransformation rate; the addition of Zn^2＋ improve the biotransformation rate from 8% to 29% ,with an increase of 263% ; the effect of Fe^3＋ exhibited a greater promotion on the coal transformation and the biotransformation rate have been increased by 184%. Moreover, the total protein content in bacterial transformation liquid showed a positive correlation with the influence tendency of metal ions on coal transformation rate.