生物活性物质的电致化学发光检测

电致化学发光是通过电极上直接或间接发生的电化学反应而产生的一种化学发光,因此电致化学发光检测是在化学发光和电化学基础上发展起来的一种新的分析技术。电致化学发光检测技术不但保留了化学发光分析和电化学分析固有的优点,同时还具有其自身的优点,如所发生的化学发光反应易于控制;方法更灵敏,更具有选择性;可以获得更多的化学信息;扩大了化学发光方法可检测的范围;更易于与现代分离技术联用。生物体中很多生物活性物质具有电活性,因此用现代电化学技术研究其电化学行为具有重要的理论意义和实际应用价值。生物体中的生物活性物质通常浓度很低,并且成分复杂,因此分离检测生物体中生物活性物质非常困难。由于电致化学发光检测具有灵敏度高,选择性好的特点,无疑是检测生物体中生物活性物质的强有力工具,如果它能与HPLC、CE及FIA等现代分离技术相结合,将表现出更加强大的生命力。

The Electrochemiluminescent Detection of Bioactive Compounds

Electrochemiluminescence (ECL) can be described as chemiluminescence (CL) produced directly or indirectly as a result of electrochemical reaction. ECL has been developed to be a new analytical method based on CL and electrochemistry. Therefore, it retains the advantages those are inherent in conventional CL and electrochemical methodology. ECL should potentially offer many additional advantages as an analytical technique, for example: CL reaction can be easier to control; better sensitivity and selectivity can be obtained; more information can be obtained, extending the range of analytes detectable with CL reaction, ECL technique can be coupled with the modern separation techniques. Many bioactive compounds in living body are electroactive, therefore, the investigation of their electrochemical behaviors using the modern electrochemical technique are very significative of theory and application. In general, the concentration of the bioactive compounds in living body is very low and suffered from serious interferences, hence it is very difficult to separate and detect the bioactive compounds in living body. Undoubtedly ECL is a powerful tool for detection of the bioactive compounds in living body due to its high sensitivity and selectivity, particularly if it can be coupled with the modern separation techniques, such as HPLC, CE and FIA. In this review, the recent progresses in ECL detection of bioactive compounds are evaluated.