| 农杆菌介导的ACC氧化酶反义基因转化甜瓜品种河套蜜瓜的研究 |
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| 引用本文： | 郝金凤,财音青格乐,方天祺,哈斯阿古拉.农杆菌介导的ACC氧化酶反义基因转化甜瓜品种河套蜜瓜的研究[J].内蒙古大学学报(自然科学版),2006,37(1):54-57. |
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| 作者姓名： | 郝金凤 财音青格乐 方天祺 哈斯阿古拉 |
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| 作者单位： | 内蒙古大学生命科学学院生物工程中心,内蒙古自治区牧草与特色作物生物技术重点实验室,呼和浩特,010021 |
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| 基金项目： | 中国科学院资助项目;国家高技术研究发展计划(863计划) |
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| 摘 要： | 将从甜瓜品种河套蜜瓜中克隆的ACC氧化酶基因cDNA片段反向构建到植物表达载体pROK II中,获得重组质粒pRACO 1,用三亲融合法将其导入根癌农杆菌LBA 4404中,转化河套蜜瓜子叶外植体,在芽诱导培养基M S IAA 0.8 m g/L BA 1.0 m g/L ABA 0.26 m g/L C ef 500 m g/L K an 75 m g/L中诱导生芽,待芽长到2cm左右转入M S IBA 0.5 m g/L C ef 300 m g/L K an 50 m g/L的培养基中诱导生根,1~2周后可诱导产生大量的根,形成完整的转化小植株.经PCR检测证明,目的基因已整合到河套蜜瓜的基因组中.
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| 关 键 词： | 甜瓜 ACC氧化酶 反义基因 转化 |
| 文章编号： | 1000-1638(2006)01-0054-04 |
| 收稿时间： | 2004-11-24 |
| 修稿时间： | 2005-06-12 |
Genetic Transformation of the ACC Oxidase Antisense Gene to Cucumis melo L.cv. Hetao Via Agrobacterium Tumefaciens |
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| HAO Jin-feng,Caiyin Qinggele,FANG Tian-qi,Hasi Agula.Genetic Transformation of the ACC Oxidase Antisense Gene to Cucumis melo L.cv. Hetao Via Agrobacterium Tumefaciens[J].Acta Scientiarum Naturalium Universitatis Neimongol,2006,37(1):54-57. |
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| Authors: | HAO Jin-feng Caiyin Qinggele FANG Tian-qi Hasi Agula |
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| Abstract: | The cDNA of the ACC oxidase gene cloned from melon Hetao was constructed reversedly into plant expression vector pROKII.The recombinant plasmid pRACO1 was introduced into melon Hetao cotyledon explants by Agrobacterium-mediated transformation.After shoot inducing culture and root inducing culture,the regenerated plantlets were obtained from the cotyledons on MS medium with 0.5 mg/L IBA,50 mg/L kanamycin and 300 mg/L cefotaximine.The PCR detection showed that the antisense gene of ACC oxidase has been integrated into melon cultivar Hetao genome. |
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| Keywords: | Cucumis melo ACC oxidase antisense gene transformation |
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