| 正交设计法在白三叶组织培养中的应用 |
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| 引用本文： | 张德炎 李喜文. 正交设计法在白三叶组织培养中的应用[J]. 东北师大学报(自然科学版), 1998, 0(1): 40-45 |
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| 作者姓名： | 张德炎 李喜文 |
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| 作者单位： | 东北师范大学遗传与细胞研究所，湖北省咸宁教育学院，青海师范大学生物系 |
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| 摘 要： | 以白三叶的叶片为外植体，采用正交设计法，对愈伤组织生长和细胞悬浮培养的培养基进行了优化筛选。实验结果表明，白三叶叶片愈伤组织生长的最佳培养基配方为ＭＢ＋０．５ｍｇ／Ｌ ２，４－Ｄ＋０．２ｍｇ／Ｌ ＮＡＡ＋１ｍｇ／Ｌ ＢＡ＋２ｍｇ／Ｌ甘氨酸＋１００ｍｇ／Ｌ肌醇＋３％蔗糖＋０．７％琼脂；细胞悬浮培养的最佳培养基的配方为ＭＢ＋４ｍｇ／Ｌ ２，４－Ｄ＋０．２ｍｇ／Ｌ ＮＡＡ＋０．５ｍｇ／Ｌ ＢＡ＋２ｍｇ／
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| 关 键 词： | 白三叶 正交设计 愈伤组织 |
Application of Orthogonal Design in Tissue Culture of White Clover(Trifolium repens) |
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| Zhang Deyan Li Xiwen Li Jianmin Xing Miao. Application of Orthogonal Design in Tissue Culture of White Clover(Trifolium repens)[J]. Journal of Northeast Normal University (Natural Science Edition), 1998, 0(1): 40-45 |
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| Authors: | Zhang Deyan Li Xiwen Li Jianmin Xing Miao |
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| Abstract: | Leaves of white clover (Trifolium repens) were used as the explants in inducement of calli. The optimum media for the callus growth and suspension cell culture were screened out by using orthogonal design. The results of the test indicate that the optimum medium for callus growth is MB+0 5 mg/L 2,4 D+ 0 2 mg/L NAA+1 mg/L BA+2 mg/L Glycine+100 mg/L Inositol+3% Sucrose+0 7% Agar;and that the optimum medium for the suspension cell culture is MB+4 mg/L 2,4 D+ 0 2 mg/L NAA+0 5 mg/L BA+2 mg/L Glycine+8% Sucrose,and 12 25 mg suspension culture product(dry weight) in 15 mL medium. |
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| Keywords: | callus orthogonal design embryogenic cell suspension culture Trifolium repens |
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