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The new sideway of CNTF signal transduction pathway |
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| Authors: | Jin Yan Cheng He Changlin Lu Chenghai Wang Xuan Bao Tieshan Tang Xiuying Huang Fangzhen Sun |
| Institution: | (1)Department of Neurobiology, Second Military Medical University ,Shanghai 200433 ,China; (2)Department of Psychology, Second Military Medical University, Shanghai ,Shanghai 200433 ,China; (3)Shanghai Brain Institute, Chinese Academy of Sciences, Shanghai ,Shanghai 200031 ,China; (4)Laboratory of Molecular Developmental Biology, Institute of Developmental Biology, Chinese Academy of Sciences ,Beijing 100080 ,China |
| Abstract: | The action of ciliary neurotrophic factor (CNTF) on intercellular free Ca2+ concentrations Ca2+]i induced by glutamate (Glu) in primary cultured hippocampal neurons were detected with Fura2/AM, a Ca2+-sensitive fluorophore, and the morphological influence of G-protein on it was objected. Glu could induce rapid increase of Ca2+]i in hippocampal neurons. CNTF had no significant action on Ca2+]i in resting hippocampal neurons. However, after incubation of CNTF for 5 min, the increase of Ca2+]i in hippocampal neurons rapidly induced by Glu was inhibited. Pretussis toxin (PTX)-sensitive G protein could block the action. These results indicate that a new non-genomic rapid sideway might exist in the upper stream of CNTF signal transduction pathway, which was related to Ca2+ signal transduction. Keywords: |
| Keywords: | ciliary neurotrophic factor glutamate intercellular free Ca2+ concentration signal transduction hippocampal neuron |
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