| Silica-Based Solid Phase Extraction of DNA on a Microchip |
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| 引用本文： | 陈晓芳 沈科跃 刘鹏 郭旻 程京 周玉祥. Silica-Based Solid Phase Extraction of DNA on a Microchip[J]. 清华大学学报, 2004, 9(4): 379-383,405 |
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| 作者姓名： | 陈晓芳 沈科跃 刘鹏 郭旻 程京 周玉祥 |
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| 作者单位： | [1]DepartmentofBiologicalSciencesandBiotechnology,TsinghuaUniversity,Beijing100084,China [2]NationalEngineeringResearchCenterforBeijingBiochipTechnology,18LifeScienceParkway,ChangpingDistrict,Beijing102206,China |
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| 基金项目： | the National Key Basic Research and Develop-ment (973) Program of China (No. G19990166) and the Na-tional High-Tech Research and Development (863) Program of China (No. 2002AAZZ2011) |
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| 摘 要： | Micro total analysis systems for chemical and biological analysis have attracted much attention. However, microchips for sample preparation and especially DNA purification are still underdeveloped. This work describes a solid phase extraction chip for purifying DNA from biological samples based on the adsorption of DNA on bare silica beads prepacked in a microchannel. The chip was fabricated with polydimethylsiloxane. The silica beads were packed in the channel on the chip with a tapered microchannel to form the packed bed. Fluorescence detection was used to evaluate the DNA adsorbing efficiency of the solid phase. The polymerase chain reaction was used to evaluate the quality of the purified DNA for further use. The extraction efficiency for the DNA extraction chip is approximately 50% with a 150-nL extraction volume, Successful amplification of DNA extracted from human whole blood indicates that this method is compatible with the polymerase chain reaction.
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| 关 键 词： | DNA复制 样本预备 固相提取物 硅石 微芯片 荧光探测 生物医学 |
Silica-Based Solid Phase Extraction of DNA on a Microchip |
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| CHEN Xiaofang,,SHEN Keyue,,LIU Peng ,,GUO Min,CHENG Jing,,ZHOU Yuxiang ,. Silica-Based Solid Phase Extraction of DNA on a Microchip[J]. Tsinghua Science and Technology, 2004, 9(4): 379-383,405 |
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| Authors: | CHEN Xiaofang SHEN Keyue LIU Peng GUO Min CHENG Jing ZHOU Yuxiang |
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| Affiliation: | CHEN Xiaofang1,2,SHEN Keyue1,2,LIU Peng 1,2,GUO Min2,CHENG Jing1,2,ZHOU Yuxiang 1,2,** 1.Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China,2.National Engineering Research Center for Beijing Biochip Technology,18 Life Science Parkway,Changping District,Beijing 102206,China |
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| Abstract: | Micro total analysis systems for chemical and biological analysis have attracted much attention. However, microchips for sample preparation and especially DNA purification are still underdeveloped. This work describes a solid phase extraction chip for purifying DNA from biological samples based on the adsorption of DNA on bare silica beads prepacked in a microchannel. The chip was fabricated with poly-dimethylsiloxane. The silica beads were packed in the channel on the chip with a tapered microchannel to form the packed bed. Fluorescence detection was used to evaluate the DNA adsorbing efficiency of the solid phase. The polymerase chain reaction was used to evaluate the quality of the purified DNA for further use. The extraction efficiency for the DNA extraction chip is approximately 50% with a 150-nL extraction volume. Successful amplification of DNA extracted from human whole blood indicates that this method is compatible with the polymerase chain reaction. |
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| Keywords: | DNA purification sample preparation solid phase extraction microchip silica |
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