A pH-activatable fluorescent aptamer probe for imaging of target cancer cells |
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| Authors: | Yan Zhou Boshi Fu Heng Xiao |
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| Institution: | 1.Department of Gastroenterology,Zhongnan Hospital of Wuhan University,Hubei,China;2.College of Chemistry and Molecular Sciences,Wuhan University,Hubei,China;3.Research Center for Tissue Engineering and Regenerative Medicine, Union Hospital,Tongji Medical College of Huazhong University of Science and Technology,Hubei,China |
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| Abstract: | Early cancer diagnosis in molecular level shows great promise in relevant prevention and clinical treatment. Herein, we developed a novel method to detect and evaluate biomarkers on cancer cell surface. Based on the excellent selectivity of AS1411 aptamer to targeted nucleolin-overexpressed cells, we used a fluorescent probe with a pH-sensitive function to label the AS1411 aptamer modified with azide by click-reaction. The spectral characteristics of fluorophore naphthalimide has a good pH dependence. By this way, nucleolin-overexpressed cell could be discriminated from normal cell. Further, this strategy could also discriminate the breast cancer tissue from the adjacent benign tissue in formalin-fixed and parrffin- embedded (FFPE) tissue specimens. It is considered that our method has the potential to be applied in medical detection. |
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