| 噬菌体筛选技术筛选与联萘酚(BINOL)相结合的抗体 |
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| 引用本文： | 张辉林,贺学礼. 噬菌体筛选技术筛选与联萘酚(BINOL)相结合的抗体[J]. 河北大学学报(自然科学版), 2007, 27(1): 79-82. DOI: 10.3969/j.issn.1000-1565.2007.01.021 |
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| 作者姓名： | 张辉林 贺学礼 |
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| 作者单位： | 河北大学,生命科学学院,河北,保定,071002 |
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| 摘 要： | 利用噬菌体展示技术筛选只结合1种对应体的BINOL抗体,建立人类单链抗体(scFv)噬菌体文库--Griffin.1文库,以固相化抗原淘筛抗体库,ELISA鉴定噬菌体抗体.从经过4轮富集的次级抗体库中挑选到噬菌体克隆,用该克隆制备的单链抗体阳性克隆,经ELISA证实具有良好的抗原特异性.从人类scFv噬菌体文库中获得抗BINOL的特异性抗体.
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| 关 键 词： | 噬菌体文库展示技术 抗体库 单链抗体 BINOL 配位体选择 |
| 文章编号： | 1000-1565(2007)01-0079-04 |
| 修稿时间： | 2006-07-10 |
Selection and Dinding Studies of Antibodies with Affinity Towards BINOL |
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| ZHANG Hui-lin,HE Xue-li. Selection and Dinding Studies of Antibodies with Affinity Towards BINOL[J]. Journal of Hebei University (Natural Science Edition), 2007, 27(1): 79-82. DOI: 10.3969/j.issn.1000-1565.2007.01.021 |
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| Authors: | ZHANG Hui-lin HE Xue-li |
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| Abstract: | Selection and binding studies of antibodies with affinity towards enantioselective BINOL.Set up human scFv phage display antibody library,Griffin.1library,using solid phase antigen with the new bio-technique of phage display and biopanning,ELISA assay antibody.The positive clones were selected after 4 times biopanning,ELISA assay with scFv from this clones testify affinity towards enantioselective BINOL.Succeed to use human scFv phage display antibody library to get antibody affinity towards enantioselective BINOL. |
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| Keywords: | phage display antibody library scFv BINOL enantioselective |
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