| 水稻叶片中可溶性蛋白质提取介质的比较 |
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| 引用本文： | 应奇才,钟国庆,邱明. 水稻叶片中可溶性蛋白质提取介质的比较[J]. 杭州师范学院学报(自然科学版), 2004, 3(1): 34-36 |
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| 作者姓名： | 应奇才 钟国庆 邱明 |
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| 作者单位： | 1. 杭州师范学院,生化与分子生物学重点实验室,浙江,杭州,310012;杭州师范学院,医学院,浙江,杭州,310012
2. 杭州师范学院,生化与分子生物学重点实验室,浙江,杭州,310012 |
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| 基金项目： | 浙江省自然科学基金(编号:302061)，中国水稻科学发展基金(编号:0003239)。 |
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| 摘 要： | 对影响紫外测定可溶性蛋白质含量的二个因素(叶绿素和石英砂)以及水稻叶片可溶性蛋白质的4种提取介质进行了比较分析,结果表明:叶绿素和石英砂对紫外测定可溶性蛋白质含量有明显影响,用0 1mol LpH6.8Tris HCl缓冲液提取的可溶性蛋白质得率最高。
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| 关 键 词： | 可溶性蛋白质 含量测定 提取 |
| 文章编号： | 1008-9403(2004)01-0034-03 |
| 修稿时间： | 2003-09-27 |
A comparison of the media of extracting soluble protein from rice leaf |
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| YING Qi-cai. A comparison of the media of extracting soluble protein from rice leaf[J]. Journal of Hangzhou Teachers College(Natural Science), 2004, 3(1): 34-36 |
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| Authors: | YING Qi-cai |
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| Affiliation: | YING Qi-cai~ |
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| Abstract: | By analysing the two factors(Chlorophyll and Quarts sand) affecting the determination of soluble protein through UV method and comparing the effect of the four buffers on the extracting content of soluble protein from rice leaf, we found that Chlorophyll and Quarts sand has some effect and 0.1 mol/L pH6.8 Tris-HCl buffer is the best for extracting the soluble protein from rice leaf. |
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| Keywords: | soluble protein measure the content extracting |
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