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| 高效毛细管电泳安培法测定麦冬中薯蓣皂甙元的含量 | |
| 引用本文： | 黄宝美,姚程炜,莫金垣,杜世章,边清泉,王志国.高效毛细管电泳安培法测定麦冬中薯蓣皂甙元的含量[J].中山大学学报(自然科学版),2010,49(2). |
| 作者姓名： | 黄宝美 姚程炜 莫金垣 杜世章 边清泉 王志国 |
| 作者单位： | 1. 绵阳师范学院化学与化学工程学院,四川,绵阳,621000 2. 中国空气动力研究与发展中心设备设计及测试技术研究所,四川,绵阳,622653 3. 中山大学化学与化学工程学院,广东,广州,510275 |
| 基金项目： | 四川省教育厅科研资助项目 |
| 摘 要： | 建立了麦冬中薯蓣皂甙元含量的高效毛细管电泳安培检测方法。着重探讨了缓冲溶液种类、浓度、酸碱度,工作电位,有机改性剂及其操作电压、进样时间等对检测的影响。Na2B4O7-NaOH(Na2B4O7浓度为30mmol.L-1)为缓冲液,工作电位为0.4V,φ为7%甲醇为有机添加剂,进样时间为5 s,在15 kV分离高压,pH=9.5的碱性条件下柱端安培法检测了薯蓣皂甙元的含量。该法的线性范围为:100~1 mg.L-1,线性方程为Y=17 224+8 722c,相关系数r=0.999 5,检出限为0.1 mg.L-1。 |
| 关 键 词： | 毛细管电泳 薯蓣皂甙元 安培检测 |
| 收稿时间： | 2009-07-28; |
Determination of Diosgenin in Radix Ophiopogonis with Amperometric Detection by Capillary Electrophoresis |
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| HUANG Baomei,YAO Chengwei,MO Jinyuan,DU Shizhang,BIAN Qingquan,WANG Zhiguo.Determination of Diosgenin in Radix Ophiopogonis with Amperometric Detection by Capillary Electrophoresis[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2010,49(2). | |
| Authors: | HUANG Baomei YAO Chengwei MO Jinyuan DU Shizhang BIAN Qingquan WANG Zhiguo |
| Institution: | HUANG Baomei1,YAO Chengwei2,MO Jinyuan3DU Shizhang1,BIAN Qingquan1,WANG Zhiguo1(1.Chemistry , Chemical Engineering,Mianyang Normal University,Mianyang 621000,China,2.Facility Design , Instrumentation Institute of CARDC,Mianyang 622653,3.School of Chemistry , Chemical Engineering,Sun Yat-sen University,Guangzhou 510275,China) |
| Abstract: | Diosgenin in radix ophiopogonis was determined by capillary electrophoresis with amperometric detection(CE-AD).The affected conditions,such as the running buffer pH value(pH),the types of running buffer,the concentration of running buffer,the detection potential,the applied voltage and the sampling time were investigated.The optimized condition was in a running buffer composed of 30 mmol/L Na2B4O7 buffer at a pH 9.5,separation voltage at 15 kV,injection time for 5 s,organic additive for 7% methanol and dete... |
| Keywords: | capillary electrophoresis diosgenin amperometric detection |
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