| 发状念珠藻中麦芽寡糖基海藻糖水解酶基因的克隆和序列分析 |
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| 引用本文： | 何靓, 吴双秀, 沈蓉蓉, 王全喜.发状念珠藻中麦芽寡糖基海藻糖水解酶基因的克隆和序列分析[J].上海师范大学学报(自然科学版),2011,40(2):202-206. |
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| 作者姓名： | 何靓 吴双秀 沈蓉蓉 王全喜 |
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| 作者单位： | 上海师范大学生命与环境科学学院,上海,200234 |
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| 基金项目： | 上海市基础研究重点项目(03JC14057); 上海市教委科研项目(05DZ29); 上海市教育委员会重点学科建设项目(J50401); 上海师范大学细胞生物学校级重点学科(DZL808) |
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| 摘 要： | 从发状念珠藻(Nostoc flaglliforme)中克隆出麦芽寡糖基海藻糖基水解酶MTHase的基因TreZ,对该基因核苷酸序列测序表明开读框全长1848 bp,编码的蛋白质有615个氨基酸.根据基因序列预测的TreZ氨基酸序列具有MTHase的催化功能保守区.发菜TreZ和TreY与点形念珠藻(Nostoc punctiforme)的TreZ和TreY有很高的同源性.结果初步表明:可以从发状念珠藻中克隆到麦芽寡糖基海藻糖水解酶基因TreZ,为进一步研究海藻糖代谢在发状念珠藻抗逆机理的工作奠定了理论和实验基础.
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| 关 键 词： | 发状念珠藻 麦芽寡糖基海藻糖水解酶 基因克隆 序列分析 |
Cloning and sequence analysis of maltooligosyltrehalose trehalohydrolase gene from nostoc flagelliforme |
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| HE Liang,WU Shuang-xiu,SHEN Rong-rong,WANG Quan-xi.Cloning and sequence analysis of maltooligosyltrehalose trehalohydrolase gene from nostoc flagelliforme[J].Journal of Shanghai Normal University(Natural Sciences),2011,40(2):202-206. |
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| Authors: | HE Liang WU Shuang-xiu SHEN Rong-rong WANG Quan-xi |
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| Institution: | HE Liang,WU Shuang-xiu,SHEN Rong-rong,WANG Quan-xi(College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China) |
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| Abstract: | A ORF fragment of TreZ gene encoding the maltooligosyl trehalose trehalohydrolase was cloned from Nostoc flaglliforme.The nucleotide sequence analysis revealed that the open reading frame length of the TreZ gene is 1848 bp,encoding a protein with 615 amino acids.The sequence analysis showed that NfTreZ gene and NfTreY gene were highly homologous to those of Nostoc Punctiforme and NfTreZ-encoded enzyme also had the conserved catalytic function of maltooligosyl trehalose trehalohydrolase.The results showed th... |
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| Keywords: | nostoc flaglliforme maltooligosyltrehalose trehalohydrolase cloning sequence analysis |
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