| “黑农35”和“黑生101”两种大豆品种胚尖再生体系的建立 |
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| 引用本文： | 张红梅, 王帆, 任永刚, 李建粤.“黑农35”和“黑生101”两种大豆品种胚尖再生体系的建立[J].上海师范大学学报(自然科学版),2011,40(2):186-190. |
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| 作者姓名： | 张红梅 王帆 任永刚 李建粤 |
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| 作者单位： | 1. 上海师范大学生命与环境科学学院,上海,200234
2. 第二军医大学流行病学教研室,上海,200433 |
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| 基金项目： | 上海市教育委员会重点学科建设项目资助(J50401) |
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| 摘 要： | 以高蛋白大豆品种"黑农35"和"黑生101"为供试品材料,采用在分化及伸长培养基中分别添加0.2 mg/L 6-BA和0.2 mg/L IBA浓度激素配比,建立了胚尖再生体系,并分别在芽诱导、不定芽伸长和生根3个环节对两种大豆品种进行了比较.结果显示:"黑农35"大豆芽诱导率高于"黑生101"大豆22.15%,两者在已出芽外植体上的平均出芽数比较接近;"黑农35"大豆不定芽伸长速度大于"黑生101"大豆,同时"黑农35"大豆大于1 cm的芽个数也多于"黑生101"大豆;"黑生101"大豆生根率以及根系生长状态好于"黑农35"大豆.本研究为今后以"黑农35"和"黑生101"大豆为材料进行遗传转化研究奠定了基础.
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| 关 键 词： | 大豆 “黑农35” “黑生101” 胚尖 再生体系 |
The establishment of embryonic tip regeneration system for soybeans Hei-Nong35 and Hei-Sheng101 |
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| ZHANG Hong-mei,WANG Fan,REN Yong-gang,LI Jian-yue.The establishment of embryonic tip regeneration system for soybeans Hei-Nong35 and Hei-Sheng101[J].Journal of Shanghai Normal University(Natural Sciences),2011,40(2):186-190. |
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| Authors: | ZHANG Hong-mei WANG Fan REN Yong-gang LI Jian-yue |
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| Institution: | ZHANG Hong-mei1,WANG Fan2,REN Yong-gang1,LI Jian-yue1(1.College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234,China,2.Department of Epidemiology,Second Military Medical University,Shanghai 200433,China) |
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| Abstract: | In order to establish soybean embryonic tip regeneration system,Hei-Nong 35 and Hei-Sheng 101 soybean with high protein were tested in this research.6-BA(0.2 mg/L)and IBA(0.2 mg/L)were added in the differentiation and elongation medium.Then the two soybean varieties were compared from three aspects,including shoot induction,elongation and radication.The results showed the shoot induction rate of Hei-Nong 35 soybean was 22.15% higher than Hei-Sheng 101,but in terms of the average number of budding from buddi... |
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| Keywords: | soybean Hei-Nong 35 Hei-Sheng 101 embryonic tip regeneration system |
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