两种新生大鼠皮质发育障碍模型的差异基因表达谱研究

目的研究两种不同的方法构建皮质发育障碍(DCDs)动物模型中的共同差异表达基因,为进一步研究DCDs形成机制提供基因水平筛选的研究平台。方法用两种方法制备DCDs模型:①射线损伤模型(射线组):采用剂量为1.45 Gy的γ射线照射妊娠15 d的SD大鼠制作子代大鼠DCDs模型;②卡莫司汀(BCNU)药物损伤模型(药物组):妊娠15 d的SD大鼠,腹腔注射BCNU制作子代大鼠DCDs模型。同时设正常对照组。对两种DCDs模型的子代新生鼠(P0)全脑做基因芯片扫描,结果与正常对照组比较,获得两种模型共有的差异基因。结果射线组大鼠与正常比较得到170个差异基因,其中25个上调,145个下调;药物组大鼠与正常比较得到259个差异基因,其中67个上调,192个下调。两组重合的基因共54个,其中3个上调,51个下调。结论皮质发育障碍是一个复杂的病理过程,本实验运用基因芯片技术,对两种不同方法构建的DCDs模型进行了研究,获得两种DCDs模型共有的基因差异表达谱,为进一步研究DCDs形成机制提供了分子生物学平台。

Researches on Differential Gene Expression Profile of Two Neonatal Rat Models for Disorders of Cortical Development

Objective Two methods for disorders of conical development （DCDs） rat models are used to compare the differentially expressions of the common genes for further investigation of the pathogenesis of DCDS with gene screening. Methods There＇re two methods for building the DCDs models：（1） ray damage model （ray group） ：1.45 Gy γ-ray is used to irradiate 15-day pregnant SD rats, in order to get the fillar generation for the DCDs models;（1） Carmustine （BCNU） injury models （drug group） ：intraperitoneal injection of BCNU are given to 15-day pregnant SD rats. Meanwhile, a normal group is needed. Affymetrix gene chips are applied to the brains of the two illar generations in the morbid group for the common changed genes of the DCDS models compared with the normal control group. Results There are 170 changed genes, with 25 are upregulated and 145 downregnlated in ray group,and 259,with 67 upregulated, and 192 downregulated in drug group,in which 54 genes with 3 upregulated 51 downregulated are coincident. Conclusion DCDs is a complex pathologic process. Two genechips techniques are use to study the two DCDs models. The common changed genes are obtained for the useful clues to investigate the molecular mechanisms of DCDs.