四羧基钴酞菁催化氧化邻苯二胺测定过氧化氢

在pH值为9.0的Britton-Robinson缓冲溶液中,以四羧基钴酞菁作为过氧化物模拟酶催化过氧化氢与邻苯二胺的反应,建立一种测定微量过氧化氢的新方法。结果显示,反应的产物2,3-二氨基吩嗪在421nm处有一最大吸收峰,其吸光度值随过氧化氢加入量的增加而增加,吸光度差值与过氧化氢浓度在0～2.07×10-4mol/L范围内呈线性关系,检出限为1.36×10-5mol/L。该方法简单,适用于微量过氧化氢含量的测定。

Determination of Hydrogen Peroxide Based on the Oxidation Reaction of o-Phenylenediamine Using Tetracarboxy Cobalt Phthalocyanine as Catalyst

In Britton-Robinson buffer solution of pH 9.0,the reaction between ophenylenediamine（OPDA） and hydrogen peroxide was catalyzed by tetracarboxy cobalt phthalocyanine,which provided a new method for the determination of hydrogen peroxide.The maximum absorption of product（2,3-Diaminophenazine） is 421 nm.As the amount of hydrogen peroxide is increase,the intensity of absorption increased.The calibration graphs of hydrogen peroxide determination was linear over the range of 0～2.07×10^-4mol/L with a detection limit of 1.36×10^-5mol/L.The method is simple and can be applied to determine samples containing microamount of H2O2.