| 几种提取苔藓植物总RNA方法的比较 |
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| 引用本文： | 张梅娟,沙伟,都艳霞.几种提取苔藓植物总RNA方法的比较[J].齐齐哈尔大学学报(自然科学版),2009,25(1). |
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| 作者姓名： | 张梅娟 沙伟 都艳霞 |
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| 作者单位： | 齐齐哈尔大学,生命科学与工程学院,黑龙江,齐齐哈尔,161006 |
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| 基金项目： | 国家自然科学基金，黑龙江省科技厅农业攻关项目，黑龙江省教育厅海外学人合作项目 |
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| 摘 要： | 将3种RNA提取方法加以比较,以期获得提取不同生境毛尖紫萼藓(Grimmia pilifera P.Beauv)总RNA的最适方法.经比较,改进后的SDS法更适合于毛尖紫萼藓总RNA的提取.此方法简单快捷,所得RNA完整性好,纯度高,试验稳定性好,可用于RT-PCR、基因克隆等进一步分子生物学研究.
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| 关 键 词： | 毛尖紫萼藓 总RNA提取 SDS法 CTAB法 Trizol法 |
Comparation of methods of total RNA extracted from bryophyte plant |
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| ZHANG Mei-juan,SHA Wei,DU Yan-xia.Comparation of methods of total RNA extracted from bryophyte plant[J].Journal of Qiqihar University(Natural Science Edition),2009,25(1). |
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| Authors: | ZHANG Mei-juan SHA Wei DU Yan-xia |
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| Institution: | College of Life Science and Engineering;Qiqihar University;Heilongjiang Qiqihar 161006;China |
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| Abstract: | In this paper,with Grimmia pilifera P.Beauv of different habitats as materials,it optimized and compared three methods of total RNA,with a view to obtaining the optimal method of RNA.Comparing to the previous methods,the modified method of quick SDS was more sutiable for Grimmia pilifera P.Beauv.It was simple,fast,high purity and good integration,as well as of stable performance.Furthermore,the total RNA could meet the needs of most molecular biology operation including RT-PCR and gene cloning,and so on. |
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| Keywords: | Grimmia pilifera P Beauv Total RNA extraction SDS CTAB Trizol |
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