Murine primitive neuroectodermal tumor: nucleolar isolation and RNA polymerase inhibition |
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Authors: | D. E. Slagel W. Kauffman |
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Affiliation: | (1) Laboratory for Neurochemistry, Division of Neurosurgery, Department of Surgery, University of Kentucky College of Medicine, 40506 Lexington, Kentucky, USA |
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Abstract: | Summary A nucleolar isolation procedure was developed for the murine primitive neuroectodermal tumor originally induced with methylcholanthrene. This procedure utilizes sonication for breakage of the nuclei at a magnesium concentration which disperses the extranucleolar chromatin. The nucleoli retain RNA synthetic activity which is inhibited to about 90% by actinomycin D and the chemotherapeutic drug adriamycin.Acknowledgments. This work was supported in part by USPHS Grant CM-67058. We wish to thank Dr William Brooks for his generous support of this project. |
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