喜树果内生真菌的分离与鉴定 |
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引用本文: | 陈贤兴,陈析丰,南旭阳,何献武. 喜树果内生真菌的分离与鉴定[J]. 河南科学, 2003, 21(4): 431-433 |
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作者姓名: | 陈贤兴 陈析丰 南旭阳 何献武 |
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作者单位: | 1. 温州师范学院生物与环境科学系,浙江,温州,325027 2. 温州出入境检验检疫局,浙江,温州,325027 |
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基金项目: | 温州市科技局科技发展计划项目(S2002A009) |
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摘 要: | 从喜树(CamptothecaacuminataDecne.)果实中分离、纯化得到5株内生真菌,经初步鉴定,分别属于半知菌亚门的匙孢霉属(MystrosporiumCda.)、鲜壳孢属(ZythiaFr.)及无孢类群(MyceliaSterilia)。其内生真菌经摇瓶培养后,采用薄层层析(TLC)法对其菌丝体提取物进行分析,初步表明WZ001菌株可能产生喜树碱(Camptothecin,CPT)的类似物。
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关 键 词: | 喜树 内生真菌 分离鉴定 |
文章编号: | 1004-3918(2003)04-0431-03 |
修稿时间: | 2003-03-04 |
Isolation and identification of endophytic fungi in the fruit of Camptotheca acuminata Decne. |
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Abstract: | Five pure endophytic fungal strains were isolated from the fruits of %Camptotheca acuminata% Decne. These strains were classified into %Mystrosporium% Cda., %Zythia% Fr. and Mycelia Sterilia of Subdivision. Cultured the strains singly in taper flask under shaking, the organic extracts of mycelium were {analyzed} with thin layer chromatography(TLC). The result preliminarily showed the strain of WZ001 might product the analog of Camptothecin(CPT). |
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Keywords: | Camptotheca acuminata Decne. endophytic fungi isolation and identification |
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