AOT／异辛烷萃取猪心细胞色素C的方法研究初探

用５０ｍｍｏｌ／ＬＡＯＴ反胶团系统在添加０．１ｍｏｌ／ＬＫＣｌ，ｐＨ７．５条件下萃取了猪心糜粗提液（Ⅰ）、ｐＨ７．５沉淀杂质蛋白后的提取液的滤液（Ⅱ）、硫酸铵（５００ｇ／Ｌ）盐析除杂蛋白的清液透析液（Ⅲ）、硫酸矮（５０ｇ／Ｌ）除杂蛋白的滤液并透析除盐的溶液（Ⅳ）、细胞色素Ｃ的三氯乙酸的沉淀物的饱和硫酸铵溶液透析除盐细胞色素Ｃ溶液（Ⅴ）中的细胞色素Ｃ。用１ｍｏｌ／ＬＫＣｌ、ｐＨ１３．０的水溶液对萃取有机相中的细胞色素Ｃ进行反萃取获得细胞色素Ｃ溶液。结果显示，ＡＯＴ反胶团系统能够将细胞色素Ｃ从猪心提取液中萃取到有机相中，并经调节离子强度和ｐＨ值又能将细胞色素Ｃ从有机相中反萃取到水溶液中。其萃取效率（％）分别为８６．９、７８．１、６３．１、４３．１和６。

Study on the Method of AOT/Isooctane for Extracting Cytochrome C from the Pork Heart Extract

This article studied the method of extracting cytC by using AOT/insooctane reversemicelle system from the first extract of pork heart,the second extract after discarding impure pro-teins and filtration in pH 7.5,the third extract after discarding impure proteins with(NH4)2SO4(500 g/L)and dialysis,the fourth extract(the dialyzed solution of the third extract after discardingimpure proteins with(NH1)2SO4 in 50 g/L and filtration),the fifth extract(it comes from the(NH4)2SO4 saturated solution of cytC-TCA sediment after dialysis)in the condition of pH 7.5,0.1mol KCl.When using 1 mol KCl pH 13.0 solution for back extract cytochrome C of organic phase,the result showed that AOT/isooctane system can extract cytC from the extract of pork heart in toorganic phase,and the back extract cytC from organic phase into water solution by changing ionicstrength and pH valtie can also be performed. The extract ratios(%)of cytC in these five solutionsare 86.9,78.1,63.1,13.1 and 6 respectively.