| 植物过氧化物酶催化联二茴香胺氧化机理探讨 |
| |
| 引用本文： | 陈泽宪,廖春花,黄开勋.植物过氧化物酶催化联二茴香胺氧化机理探讨[J].华中科技大学学报(自然科学版),2001,29(1):99-101. |
| |
| 作者姓名： | 陈泽宪 廖春花 黄开勋 |
| |
| 作者单位： | 华中科技大学化学系 |
| |
| 基金项目： | 教育部留学人员回国基金资助项目 |
| |
| 摘 要： | 用高效液相色谱法（HPLC）对大麦苗含硒过氧化物酶催化联二茴香胺-H2O2反应体系的主要氧化产物进行了分离纯化，并用UV，IR，MS和NMR进行了结构表征。实验结果表明：该体系的主要反应产物为联二茴香胺的二聚体。在此基础上，对含硒过氧化物酶催化联二茴香胺-H2O2反庆的机理作了进一步的推测。
|
| 关 键 词： | 含硒过氧化物酶 联二茴香胺 大麦苗 催化氧化机理 高效液相色谱法 |
| 文章编号： | 1000-8616(2001)01-0099-03 |
Oxidation Mechanism of O-dianisidine Catalyzed by Selenium-containing Peroxidase |
| |
| Chen Zexian,LIAO Chunhua,Huang Kaixun.Oxidation Mechanism of O-dianisidine Catalyzed by Selenium-containing Peroxidase[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,2001,29(1):99-101. |
| |
| Authors: | Chen Zexian LIAO Chunhua Huang Kaixun |
| |
| Abstract: | The H 2O 2 dependent oxidation and polymerization of O dianisidine catalyzed by selenium containing peroxidase from germinating barley are focused on. The isolation and purification of the products in the system are achieved by high pressure liquid chromatography (HPLC). The structure of major product is characterized by NMR, IR, UV and MS. The results show that under the studied condition, the product of o dianisidine H 2O 2 catalyzed by selenium containing peroxidase is a dimmer of o dianisidine. The reaction mechanism is discussed based on the product structure and the previous research results. |
| |
| Keywords: | selenium containing peroxidase o dianisidine germinating barley |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
