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分光光度法测定黄芪药材中铜、铁、锌的含量
引用本文:沈颂章,季大伟,邢福,王小燕,林锦明,何邦平.分光光度法测定黄芪药材中铜、铁、锌的含量[J].海南大学学报(自然科学版),2010,28(1):36-37.
作者姓名:沈颂章  季大伟  邢福  王小燕  林锦明  何邦平
作者单位:1. 第二军医大学,药学院
2. 第二军医大学,研究生管理大队药学本科14队,上海,200433
基金项目:第二军医大学大学生创新能力培养基金资助项目的部分内容 
摘    要:为了探讨分光光度法测定黄芪药材中Cu,Fe,Zn质量含量的可行性,笔者根据不同的金属离子与特定显色剂显色,可产生可见光吸收的现象,采用分光光度法平行测定人发标准品及黄芪中微量元素的质量含量.结果表明,黄芪中含丰富的微量元素,其中Fe质量含量最高(为173.08 mg.kg-1),Zn为75.87 mg.kg-1,Cu为17.05 mg.kg-1.分光光度法操作简便、测量快速、结果准确,易推广使用.

关 键 词:分光光度计  黄芪  Cu  Fe  Zn  测定

Determination of Copper Iron and Zinc in Radix astragali
SHEN Song-zhang,JI Da-wei,XING Fu,WANG Xiao-yan,LIN Jing-ming,HE Bang-ping.Determination of Copper Iron and Zinc in Radix astragali[J].Natural Science Journal of Hainan University,2010,28(1):36-37.
Authors:SHEN Song-zhang  JI Da-wei  XING Fu  WANG Xiao-yan  LIN Jing-ming  HE Bang-ping
Institution:1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China; 2, Graduate School of Management Pharmacy Students 14 Team,Shanghai 200433, China )
Abstract:In order to discuss the feasibility of determining the contents of Cu, Fe, Zn in Radix astragali by spectrophotometry. In the paper, based on the phenomenon that different metal ions can be colored with specific color reagent, engendering visible light absorption, spectrophotometry was adopted to determine the contents of Radix astragali and that of human hair which is used as standard substance. The results showed that Radix astragali is rich in trace element, the content of Fe was 173.08mg·kg^-1, Zn was 75.87 mg·kg^-1 ,Cu was 17.05 mg·kg^-1. These data indicated that spectrophotometry has the advantages of simple operation, high-speed and accurate, and could be used widely.
Keywords:Cu  Fe  Zn
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