| 人胎肝金属硫蛋白的分离纯化及酶联免疫吸附检测 |
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| 引用本文： | 郑军恒,茹刚,茹炳根. 人胎肝金属硫蛋白的分离纯化及酶联免疫吸附检测[J]. 北京大学学报(自然科学版), 1999, 35(2): 225-229 |
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| 作者姓名： | 郑军恒 茹刚 茹炳根 |
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| 作者单位： | 北京大学生命科学学院，北京，100871 |
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| 基金项目： | 科技部科技攻关项目;96-CO3-01-06; |
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| 摘 要： | 人胎肝经匀浆、离心、乙醇沉淀后，通过Sephadex G-75、DEAE-Sepharose Fast Flow柱层析分离，获得金属硫蛋白的两种亚型(MT-1和MT-2)。经氨基酸组成分析证明是均一的。MT-1为抗原免疫青紫兰兔制备了抗血清，以纯化的IgG建立了人MT的酶联免疫吸附检测(ELISA)方法，并给出了标准工作曲线。
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| 关 键 词： | 金属硫蛋白 酶联免疫吸附检测 分离纯化 |
| 收稿时间： | 1997-12-31 |
Purification and Enzyme-Linked Immunosorbent Assay of Human Fetal Liver Metallothionein |
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| ZHENG Junheng,RU Gang,RU Binggen. Purification and Enzyme-Linked Immunosorbent Assay of Human Fetal Liver Metallothionein[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1999, 35(2): 225-229 |
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| Authors: | ZHENG Junheng RU Gang RU Binggen |
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| Affiliation: | College of Life Sciences, Peking University, Beijing, 100871 |
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| Abstract: | After human fetal liver was homogenized,centrifuged and precipitated with ethanol,two forms of metallothionein (MT 1,MT 2) were purified by gel filtration chromatography on Sephadex G 75,ion exchange chromatography on DEAE Sepharose Fast Flow.Their purity was verified by amino acid composition analysis.Using human MT 1 as antigen,the antiserum was produced in rabbit.Then enzyme linked immunosorbent assay (ELISA) was developed for the detection and quantitative analysis of human metallothionein,and the standard curves were supplied. |
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| Keywords: | metallothionein enzyme linked immunosorbent assay (ELISA) purification and isolation |
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