| 草鱼肌肉生长抑制素cDNA克隆及序列分析 |
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| 引用本文： | 孙翰昌,黄利,丁诗华.草鱼肌肉生长抑制素cDNA克隆及序列分析[J].渝西学院学报(自然科学版),2011(1):50-54. |
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| 作者姓名： | 孙翰昌 黄利 丁诗华 |
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| 作者单位： | 重庆文理学院生命科学与技术学院;西南大学动物科技学院水产系水产科学重庆市重点实验室; |
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| 基金项目： | 重庆市教委科技项目(KJ091215); 重庆文理学院科研项目(Y2009SK68) |
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| 摘 要： | 利用RT-PCR技术,从草鱼肌肉组织中克隆出MSTN cDNA序列,长度为1764 bp,编码区为1128 bp,共编码375个氨基酸.前体蛋白包括信号肽序列、N端前肽区、蛋白酶水解位点RIRR及C端活性区(含9个保守的Cys残基).多重序列比较表明,草鱼与斑马鱼、黑头软口鲦之间的MSTN序列同源性在94%以上,与其它鱼类之间的序列同源性也较高(78.1%~82.3%),但与鸟类、哺乳类之间的序列同源性则相对较低.系统进化分析显示,不同物种间的MSTN序列同源性基本反映了它们的亲缘关系.
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| 关 键 词： | 草鱼 肌肉生长抑制素 克隆 序列分析 |
Molecular cloning and sequence analysis of myostatin cDNA from grass carp(Ctenopharyngodon idellus) |
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| Authors: | SUN Han-chang HUANG Li DING Shi-hua |
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| Institution: | SUN Han-chang1,HUANG Li2,DING Shi-hua2(1.School of Life Science and Technology,Chongqing University of Arts and Sciences,Yongchuan Chongqing 402168,China,2.Department of Fishery Sciences,College of Animal Science and Technology,Key Laboratory of Aquatic Science of Chongqing,Southwest University,Beibei Chongqing 400716,China) |
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| Abstract: | The cDNA encoding myostatin(MSTN) was cloned from the skeletal muscle of grass carp by RT-PCR method.It was 1764 bp long with a coding region of 1128 bp encoding a precursor of 375 amino acids.The precursor had a putative signal sequence,an inactive N-terminal propeptide region,a conserved proteolytic processing site(RIRR) and an active C-terminal region(containing nine conserved cysteine residues).Sequence alignment revealed that the MSTN precursor of grass carp shared high homology of more than 94% with t... |
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| Keywords: | Ctenopharyngodon idellus MSTN cloning sequence analysis |
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