Bcl-2家族蛋白和乙肝病毒x蛋白在肝癌组织中的表达和意义

应用免疫组织化学方法检测了34例肝癌组织及其相对应的癌旁组织,探讨了Bcl-2家族中七种基因(包括促凋亡基因Bak、Bad、Bid、Bax和Bcl-xs及抑凋亡基因Bcl-2、Bcl-w)和乙肝病毒三种抗原(包括HBsAg、HBcAg和HBxAg)在肝癌组织中的表达及意义,结果显示:在肝癌组织中HBsAg、HBcAg和HBxA的阳性率分别为58.8％、26.5％和76.5％、Bcl-2七种蛋白的阳性率分别为58.8％(Bak)、55.9％(Bad)、44.1％(Bid)、41.2％(Bax)、29.4％(Bcl-xs)、35.3％(Bcl-w)和41.2％(Bcl-2)。这七种Bcl-2蛋白的表达均位于肝癌细胞的胞浆,多呈弥漫性分布,少数阳性颗粒呈散在性分布,研究发现,Bcl-2家族中抑凋亡基因Bcl-w和Bcl-2在癌组织中表达的阳性率明显高于癌旁组织(P

EXPRESSION OF BCL-2 FAMILY PROTEINS AND HEPATITIS B VIRUS X PROTEIN IN HEPATOCELLULAR CARCINOMA TISSUES AND THEIR SIGNIFICANCE

In this study, 34 cases of hepatocellular carcinoma (HCC) tissues and their corresponding paracancerous ones were studied by immunohistochemical method. The expression of seven members of Bcl-2 family (including pro-apoptosis member of Bcl-2 family such as Bak, Bad, Bid, Bcl-xs and Bax, and anti-apoptosis ones such as Bcl-w and Bcl-2) and hepatitis B virus (HBV) proteins (such as, HBsAg, HBcAg and HbxAg) was detected in the tissues to texplore the significance of their expression. The results showed that the positive rates of HBsAg, HBcAg and HBxAg were 55.8% ,26.5% and 88.2%, respectively in 34 cases of HCC tissues. The positive rates of 7 proteins of Bcl-2 family were 58.8%(Bak), 58.9% (Bad), 44.1%(Bid), 41.2%(Bax),29.4%(Bcl-xs), 35.3%(Bcl-w) and 41.2%(Bcl-2), respectively in cancerous tissures. All of the 7 proteins were mainly found in the cytoplasm of the ancerous and non-cancerous cells. The positive cells were mainly distributed in diffuse pattern, and partly in scatter ones. Particularly, the positive rates of anti-apoptosis members of Bcl-2 family (Bcl-w and Bcl-2)are significantly higher in hepatoma tissues than those in paracancerous ones (P<0. 05). It suggests that the over expression of these members of Bcl-2 family maybe actively involved in the regulation on apoptosis in HCC tissues infected HBV. Therefore, it is demonstrated that the Bcl-2 family genes were closely related to the occurrence and development of HCC.