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| 低强度He- Ne 激光与丹参注射液联用对牛中性粒细胞呼吸爆发的影响 | |
| 引用本文： | 李艳玲,角建瓴,刘承宜,刘颂豪.低强度He- Ne 激光与丹参注射液联用对牛中性粒细胞呼吸爆发的影响[J].华南师范大学学报(自然科学版),2003,0(1):115-118. |
| 作者姓名： | 李艳玲 角建瓴 刘承宜 刘颂豪 |
| 作者单位： | 1. 华南师范大学信息光电子科技学院,广东广州,510631 2. 暨南大学中医学系,广东广州510632 3. 华南师范大学激光运动医学实验室,广东广州,510631 |
| 基金项目： | 国家自然科学基金 (6 0 1780 0 3,6 0 2 7812 )，广东省自然科学基金团队项目 (2 0 0 0 30 6 1)和重点项目 (2 0 0 11480 )，激光技术国家实验室开放基金，广东省“千百十工程”优秀人才培养基金 (Q0 2 0 87) |
| 摘 要： | 为了探讨低强度Ｈｅ -Ｎｅ激光与丹参注射液联合作用在细胞水平的生物效应 ,本文应用粒细胞的化学发光法1] 检测了单用低强度Ｈｅ -Ｎｅ激光或丹参注射液、低强度Ｈｅ -Ｎｅ激光与丹参注射液联用对中性粒细胞呼吸爆发的影响 ,以进一步探讨其抗缺血、缺氧性损伤的机制 .1 材料和方法1 .1 材料 趋化三肽 (Ｎ -ｆｏｒｍｙｌ-ｍｅｔｈｉｏｎｙｌ-ｌｅｕｃｙｌ-ｐｈｅｎｙｌａｌａｎｉｎｅ ,ｆＭＬＰ) ,鲁米诺 ( 5-ａｍｉｎｏ - 2 ,3-ｄｉ ｈｙｄｒｏ - 1 ,4-ｐｈｔｈａｌａｚｉｎｅｄｉｏｎｅ ,Ｌｕｍｉｎｏｌ) ,ＤｅｘｔｒａｎＴ - 50 0… |
| 关 键 词： | 低强度He－Ne激光 丹参注射液 中性粒细胞 呼吸爆发 |
| 文章编号： | 1000-5463(2003)01-0115-04 |
| 修稿时间： | 2002年2月16日 |
EFFECTS OF LOW INTENSITY He-Ne LASER IRRADIATION COMBINED WITH SALVIA MILTIORRHIZA INJECTION ON THE RESPIRATORY BURST OF BOVINE NEUTROPHILS |
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| LI Yan-ling,JIAO Jian-ling,LIU Cheng-yi,LIU Song-hao.EFFECTS OF LOW INTENSITY He-Ne LASER IRRADIATION COMBINED WITH SALVIA MILTIORRHIZA INJECTION ON THE RESPIRATORY BURST OF BOVINE NEUTROPHILS[J].Journal of South China Normal University(Natural Science Edition),2003,0(1):115-118. | |
| Authors: | LI Yan-ling JIAO Jian-ling LIU Cheng-yi LIU Song-hao |
| Institution: | LI Yan-ling1,JIAO Jian-ling2,LIU Cheng-yi3,LIU Song-hao1 |
| Abstract: | Neutrophils can generate oxygen free radicals by respiratory burst. Oxygen free radicals injury is one of the important reasons of ischemia and anoxemia. Low intensity He-Ne laser irradiation combined with Salvia miltiorrhiza injection(SM) can treat ischemia and anoxemia well in clinic, but the mechanism remains obscure. Primary study on cellular level is needed. In the paper, the effects of low intensity He-Ne laser irradiation, SM, and the combination of low intensity He-Ne laser irradiation and SM on the respiratory burst of bovine neutrophils are examined, respectively. In the study, He-Ne laser, SM or the combination of He-Ne laser and SM all can inhibit the respiratory burst of neutrophils evidently. The effect of the combination of laser and SM is more marked than that of only laser or SM. These results can help to understand the mechanism of ischemic injury. |
| Keywords: | low intensity He-Ne laser Salvia miltiorrhiza injection neutrophil respiratory burst |
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