FISH analysis of the integration patterns in transgenic rice co-transformed by microprojectile bombardment |
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Authors: | Weiwei Jin Zongyun Li Shunbin Ning Dinghou Ling Lijia Li Yunchun Song |
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Institution: | (1) Key Lab of the Ministry of Education for Plant Developmental Biology, Wuhan University, 430072 Wuhan, China;(2) South China Institute of Botany, Chinese Academy of Sciences, 510650 Guangzhou, China |
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Abstract: | Using multi-color fluorescencein situ hybridization (FISH), we localized transferredbarnase-ps1 andpHctinG DNA sequences onto chromosomes of two transgenic rice plants, named Q12 and Q13, both of which were produced by micro-projectile
bombardment. In both Q12 and Q13, each detected cell showed 2–3 signal spots on their chromosomes respectively. The signals
of bothbarnase-ps1 andpHctinG were mostly detected in the adjacent chromosomal sites in which their signals were overlapped and could be recognized by
the signal color on the metaphase chromosomes. Fiber FISH further demonstrated that the multiple copies in each of the two
DNA sequences distributed adjacently on the DNA fiber in Q13. Combined with the results of Southern hybridization, the possible
integration patterns in transgenic rice co-transformed by micro-projectile bombardment have been discussed. |
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Keywords: | FISH transgenic rice co-transformation integration pattern |
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