金匮肾气丸中三萜类成分的质量比测定研究

建立金匮肾气丸中三萜类成分的质量比测定方法.以HPLC法选用SymmetryC18柱（4.6 mm×250 mm,5μm）测定该复方中茯苓酸、熊果酸及齐墩果酸的质量比.茯苓酸质量比测定色谱条件为：检测波长为242 nm;流动相为甲醇-水-磷酸（75∶25∶0.2）;流速为1 mL/min;柱温为30℃.熊果酸及齐墩果酸的色谱条件：检测波长为215 nm;流动相为乙腈-甲醇-水-乙酸胺（62.5∶15.5∶22∶0.55）;流速为0.8 mL/min;柱温为30℃.茯苓酸、熊果酸及齐墩果酸的质量比分别为0.116 mg/g、0.287 mg/g、0.134 mg/g.结果表明,该方法简便、准确、重现性好,适用于金匮肾气丸中三萜酸类成分的定量分析.

Study on content determination of triterpene in Jinkuishenqi pill

To establish quantitative method of determination of triterpene in Jinkuishenqi pill. Determinates the content of tumulosic acid, malol and oleanolic acid by HPLC on Symmetry C18 column （4. 6 mm × 250 mm,5 μm）. The color spectrum condition of tumulosic acid are ： The mobile phase consisted of methanol - water - phosphoric acid （ 75： 25： 0. 2 ） with detected wavelength at 242 nm, flow rate at 1 mL/min, the conlum temperature is 30 ℃. The color spectrum condition of malol and oleanolic acid are ： The mobile phase consisted of acetonitrile - methanol - water - ammonium acetate （62. 5：15.5： 22： 0. 55 ）with detected wavelength at 215 nm, flow rate at 0. 8 mL/min. The conlum temperature is 30 ℃. The content of tumulosic acid, malol and oleanolic are 0. 116, 0. 287 ,0. 134 mg/g. The method is sensitive ,rapid and accurate, suitable for the determination of triterpene in Jinkuishenqi pill.