A high-sensitive detection method for carvone odor by implanted electrodes in rat olfactory bulb |
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Authors: | Liujing Zhuang Ning Hu Feng Tian Qi Dong Liang Hu Rong Li Ping Wang |
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Institution: | 1. Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China
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Abstract: | Mammalian olfactory systems have extraordinary ability to sense and identify various trace odorants.Taking advantages of cell culture and micro-fabrication technologies,olfactory cell-or tissue-based biosensor represent a promising platform for in vitro odorant detection.However,in vitro conditions lead to shortened cell/tissue survivals,and the working life of neuron chips is short.The purpose of this study is to develop an in vivo recording and analyzing method for long-term and repeatable detection of odor stimulation.In this study,we implanted penetrating micro-wire array electrode into the olfactory bulb of conscious rats to obtain odor-evoked electrophysiological activities.Then,we investigated the response of ensembles of mitral/tufted cells to stimulation with carvone at a number of concentrations in time and frequency domains.The stable,repeatable odorant responses from up to 16 neural regions could be obtained for at least 3 weeks.Further,we explored the concentration detection sensitivity limitation of developed method,and found the detection low limit of carvone was below10-10mol/L.The result demonstrates that the concentration range of in vivo odorant detection method is much wider than in vitro method. |
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Keywords: | Electrophysiology · Micro-wire arrayelectrode · Olfactory bulb ·Odorant detection ·High-sensitive |
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