| Cellular prion protein imaging analysis with aptamer-labeled Ru（bpy）32＋-doped silica nanoparticles |
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| 引用本文： | Wei Wang,Xiao Li Yan,Lei Zhan,Fei Leng,Xiao Xi Yang,Cheng Zhi Huang. Cellular prion protein imaging analysis with aptamer-labeled Ru（bpy）32＋-doped silica nanoparticles[J]. 科学通报(英文版), 2014, 59(2): 147-153. DOI: 10.1007/s11434-013-0052-1 |
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| 作者姓名： | Wei Wang Xiao Li Yan Lei Zhan Fei Leng Xiao Xi Yang Cheng Zhi Huang |
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| 作者单位： | Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences,Southwest University;College of Chemistry and Chemical Engineering, Southwest University |
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| 基金项目： | supported by the National Basic Research Program of China(2011CB933600) |
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| 摘 要： | A new type of highly selective aptamer-labeled fluorescent silica nanoparticles [Apt-tris（2,2＇-bipyri- dyl）ruthenium（II）@SiO2 NPs] were prepared through the reverse microemulsion method by using prolonged fluorescence lifetime ruthenium complexes of tris（2,2＇-bipyri- dyl）ruthenium（II） （Ru（bpy）2＋） as the source of the fluorescence for cellular prion protein imaging. Investigations showed that the newly prepared Ru（bpy）32＋@Si02 NPs possessed superior advantages of strong fluorescence, low toxicity, and easy surface modification for bioconjugation. Cell imaging experiments indicated that Apt- Ru（bpy）32＋@SiO2 NPs had great tendency to human bone marrow neuroblastoma cells （SK-N-SH cells）, since they can express large amount of prion protein on the surface of the cell, while in HeLa cells this phenomenon disappeared for the reason that HeLa cells cannot express prion proteins.
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| 关 键 词： | Ru(bpy)+@SiO NPs·Aptamer·Prion protein(PrPC)·Cell imaging |
Cellular prion protein imaging analysis with aptamer-labeled Ru(bpy) 3 2+ -doped silica nanoparticles |
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| Wei Wang,Xiao Li Yan,Lei Zhan,Fei Leng,Xiao Xi Yang,Cheng Zhi Huang. Cellular prion protein imaging analysis with aptamer-labeled Ru(bpy) 3 2+ -doped silica nanoparticles[J]. Chinese science bulletin, 2014, 59(2): 147-153. DOI: 10.1007/s11434-013-0052-1 |
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| Authors: | Wei Wang Xiao Li Yan Lei Zhan Fei Leng Xiao Xi Yang Cheng Zhi Huang |
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| Affiliation: | 1. Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China
2. College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China
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| Abstract: | A new type of highly selective aptamer-labeled fluorescent silica nanoparticles [Apt-tris(2,2′-bipyridyl)ruthenium(II)@SiO2 NPs] were prepared through the reverse microemulsion method by using prolonged fluorescence lifetime ruthenium complexes of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ ) as the source of the fluorescence for cellular prion protein imaging. Investigations showed that the newly prepared Ru(bpy) 3 2+ @SiO2 NPs possessed superior advantages of strong fluorescence, low toxicity, and easy surface modification for bioconjugation. Cell imaging experiments indicated that Apt-Ru(bpy) 3 2+ @SiO2 NPs had great tendency to human bone marrow neuroblastoma cells (SK-N-SH cells), since they can express large amount of prion protein on the surface of the cell, while in HeLa cells this phenomenon disappeared for the reason that HeLa cells cannot express prion proteins. |
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| Keywords: | Ru（bpy）32＋@SiO2 NPs - AptamerPrion protein （PrPc） - Cell imaging |
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