超临界CO2中脂肪酶催化反应的研究

This paper studies the lipase-catalyzed esterification of lauric acid and n-butanol in supercritical carbon dioxide．The method is the same as described in “Journal of Tsinghua University” (Qinghua Daxeu Xuebao)，vol.39，No.6，pp31～34，1999，by CHEN Huiqing，YANG Jichu．The effect of pressure，temperature，water content of the enzyme，concentration and type of the substrates on the reaction are evaluated．Increasing the pressure will make the hydration extend of the enzyme decrease，along with the decrease of the activity．However，the solubility of all substrates increase when turning the pressure from 8MPa to 20MPa，a higher reaction rate thus is induced．In the range of 34℃～60℃，increase of the temperature will cause a tendency of higher reaction rate．The water contents of the enzyme have a great impact on the reaction．The activity of the enzyme reaches its peak at the water mass fraction of 40%．If the concentration of n-butanol is greater than 100mmol/L，an obivous inhibition on the enzyme is observed．During each experiment，lauric acid did not inhibit the reaction．The initial rate increases with the extending of carbon chain in alcohol molecule．When the number of carbon atom is more than 4，the reaction rate lowers down.