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| 反义ACC合成酶基因导入番茄的研究 | |
| 引用本文： | 曾黎琼,张绍松,柴红梅,王敬乔,李成云,张云孙,罗云波. 反义ACC合成酶基因导入番茄的研究[J]. 云南大学学报(自然科学版), 2002, 24(5): 393-397 |
| 作者姓名： | 曾黎琼 张绍松 柴红梅 王敬乔 李成云 张云孙 罗云波 |
| 作者单位： | 1. 云南省农业科学院生物技术研究所,云南,昆明,650223 2. 云南大学,云南,昆明,650091 3. 中国农业大学,北京,100094 |
| 基金项目： | 云南省省院省校合作项目资助 (98ZB0 5 ) |
| 摘 要： | 采用农杆菌介导法将携带有反义ACC合成酶基因和NPT II的表达载体pKAC导入番茄栽培品种美国大红五星102、美国大红108、美国大红王、大红II号等中.经过卡那霉素筛选后,获得了一批抗性苗,其中有3株PCR检测为阳性.实验证明,不同激素配比对番茄子叶不定芽的诱导有一定影响,适宜的农杆菌侵染时间和浓度是番茄基因转化成功与否的关键,延迟选择有利于番茄基因转化细胞的生长. |
| 关 键 词： | 农杆菌介导法 番茄 反义ACC合成酶基因 延迟选择 |
| 文章编号： | 0258-7971(2002)05-0393-05 |
| 修稿时间： | 2002-02-16 |
Studies on transformation of antisense ACCase gene into tomatoes |
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| ZENG Li qiong ,ZHANG Shao song ,CHAI Hong mei ,WANG Jing qiao ,LI Cheng yun ,ZHANG Yun sun ,LUO Yun bo. Studies on transformation of antisense ACCase gene into tomatoes[J]. Journal of Yunnan University(Natural Sciences), 2002, 24(5): 393-397 | |
| Authors: | ZENG Li qiong ZHANG Shao song CHAI Hong mei WANG Jing qiao LI Cheng yun ZHANG Yun sun LUO Yun bo |
| Affiliation: | ZENG Li qiong 1,ZHANG Shao song 1,CHAI Hong mei 1,WANG Jing qiao 1,LI Cheng yun 1,ZHANG Yun sun 2,LUO Yun bo 3 |
| Abstract: | An expressing vector pKAC with antisense ACCase gene and NPT II was transferred into some tomato strains by agrobacterium mediated transformation.After selection culture with kanamycin,some transgenic plants of tomato were obtained.Three of them were positive by PCR analysis.The results showed three characteristics,namely,1.The inductive rate of adventitious bud of tomato cotyledon was different on various media with different hormone and concentration.2.The concentration of agrobacterium and time of agrobacterium infection were the keys to gene transformation of tomato.3.The delayed selection culture was helpful to the growth of transgenic cells. |
| Keywords: | agrobacterium mediated transformation tomato antisense ACCase gene the delayed selection culture |
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