首页 | 本学科首页   官方微博 | 高级检索  
     


Global landscape of protein complexes in the yeast Saccharomyces cerevisiae
Authors:Krogan Nevan J  Cagney Gerard  Yu Haiyuan  Zhong Gouqing  Guo Xinghua  Ignatchenko Alexandr  Li Joyce  Pu Shuye  Datta Nira  Tikuisis Aaron P  Punna Thanuja  Peregrín-Alvarez José M  Shales Michael  Zhang Xin  Davey Michael  Robinson Mark D  Paccanaro Alberto  Bray James E  Sheung Anthony  Beattie Bryan  Richards Dawn P  Canadien Veronica  Lalev Atanas  Mena Frank  Wong Peter  Starostine Andrei  Canete Myra M  Vlasblom James  Wu Samuel  Orsi Chris  Collins Sean R  Chandran Shamanta  Haw Robin  Rilstone Jennifer J  Gandi Kiran  Thompson Natalie J  Musso Gabe  St Onge Peter  Ghanny Shaun  Lam Mandy H Y  Butland Gareth  Altaf-Ul Amin M
Affiliation:Banting and Best Department of Medical Research, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College St, Toronto, Ontario M5S 3E1, Canada.
Abstract:Identification of protein-protein interactions often provides insight into protein function, and many cellular processes are performed by stable protein complexes. We used tandem affinity purification to process 4,562 different tagged proteins of the yeast Saccharomyces cerevisiae. Each preparation was analysed by both matrix-assisted laser desorption/ionization-time of flight mass spectrometry and liquid chromatography tandem mass spectrometry to increase coverage and accuracy. Machine learning was used to integrate the mass spectrometry scores and assign probabilities to the protein-protein interactions. Among 4,087 different proteins identified with high confidence by mass spectrometry from 2,357 successful purifications, our core data set (median precision of 0.69) comprises 7,123 protein-protein interactions involving 2,708 proteins. A Markov clustering algorithm organized these interactions into 547 protein complexes averaging 4.9 subunits per complex, about half of them absent from the MIPS database, as well as 429 additional interactions between pairs of complexes. The data (all of which are available online) will help future studies on individual proteins as well as functional genomics and systems biology.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号