| Sequence analysis on biological active peptides using electrospray ionizationFourier transform ion cyclotron resonance mass spectrometer |
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| 引用本文： | CHENHui XUJiaxi XINBin HEMeiyu. Sequence analysis on biological active peptides using electrospray ionizationFourier transform ion cyclotron resonance mass spectrometer[J]. 科学通报(英文版), 2005, 50(9): 853-857. DOI: 10.1007/BF02897377 |
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| 作者姓名： | CHENHui XUJiaxi XINBin HEMeiyu |
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| 作者单位： | [1]DepartmentofChemistry,PekingUniversity,Beijing100871,China [2]BeijingMassSpectrometryCenter,Beijing100080,China |
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| 摘 要： | This paper describes experience with the sequencing of synthetic segment peptides of gp41 which are used in trimeric peptides as potential peptide-based vaccines and as inhibitors to mimic the fusion state of gp41 in HIV infection using ESI/CID-FT-ICR MS. All b and y““ series ions are found in the CID spectra and the mass difference between the calculated and observed results is very slight. FT-ICR MS again shows its power in peptide sequencing, successfully helping us obtain the sequence of an unknown peptide.
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| 关 键 词： | 缩氨酸 分子序列 生物学 回旋加速器 电离作用 |
| 收稿时间： | 2004-07-06 |
Sequence analysis on biological active peptides using electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometer |
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| Chen Hui,Xu Jiaxi,Xin Bin,He Meiyu. Sequence analysis on biological active peptides using electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometer[J]. Chinese science bulletin, 2005, 50(9): 853-857. DOI: 10.1007/BF02897377 |
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| Authors: | Chen Hui Xu Jiaxi Xin Bin He Meiyu |
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| Affiliation: | (1) Department of Chemistry, Peking University, 100871 Beijing, China;(2) Beijing Mass Spectrometry Center, 100080 Beijing, China |
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| Abstract: | This paper describes experience with the sequencing of synthetic segment peptides of gp41 which are used in trimeric peptides as potential peptide-based vaccines and as inhibitors to mimic the fusion state of gp41 in HIV infection using ESI/CID-FT-ICR MS. All b and y′’ series ions are found in the CID spectra and the mass difference between the calculated and observed results is very slight. FT-ICR MS again shows its power in peptide sequencing, successfully helping us obtain the sequence of an unknown peptide. |
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| Keywords: | peptide ESI FT-ICR MS SORI-CID. |
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