| HPLC测定香椿中的有机酸和黄酮等7种活性成分 |
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| 引用本文： | 高意,周光明,张彩虹,于璐,陈军华,廖安辉. HPLC测定香椿中的有机酸和黄酮等7种活性成分[J]. 四川大学学报(自然科学版), 2017, 54(5): 1039-1044 |
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| 作者姓名： | 高意 周光明 张彩虹 于璐 陈军华 廖安辉 |
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| 作者单位： | 西南大学化学化工学院发光与实时分析教育部重点实验室,西南大学化学化工学院发光与实时分析教育部重点实验室,西南大学化学化工学院发光与实时分析教育部重点实验室,西南大学化学化工学院发光与实时分析教育部重点实验室,西南大学化学化工学院发光与实时分析教育部重点实验室,西南大学化学化工学院发光与实时分析教育部重点实验室 |
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| 基金项目： | 国家自然科学基金面上项目21277110 |
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| 摘 要： | 采用Phenomenex C18色谱柱(150mm×4.6mm,5μm)分离7种成分;流动相为甲醇-0.1%醋酸溶液,梯度洗脱;流速:0.8mL·min~(-1),紫外检测波长:290nm,柱温:35℃.建立超声辅助萃取-高效液相色谱法(HPLC)分离香椿中表儿茶素、没食子酸、儿茶素、芦丁、杨梅素、槲皮素和山奈酚7种活性成分及其含量测定的方法.结果发现7种成分在15min内均达到基线分离,线性关系良好(r0.9991,n=7),平均回收率均在99.23%~104.5%(RSD%1.26%,n=3).本实验采用超声波法提取,方法简单、快速、经济、可靠,可用于香椿的质量监控.
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| 关 键 词： | 高效液相色谱；香椿；超声萃取；黄酮；有机酸；含量测定 |
| 收稿时间： | 2016-03-23 |
| 修稿时间： | 2016-05-09 |
Determination of Six Flavonoids in Agrimonia Pilosa Ledeb by HPLC |
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| GAO Yi,ZHOU Guang-Ming,ZHANG Cai-Hong,YU Lu,CHEN Jun-Hua and LIAO An-Hui. Determination of Six Flavonoids in Agrimonia Pilosa Ledeb by HPLC[J]. Journal of Sichuan University (Natural Science Edition), 2017, 54(5): 1039-1044 |
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| Authors: | GAO Yi ZHOU Guang-Ming ZHANG Cai-Hong YU Lu CHEN Jun-Hua LIAO An-Hui |
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| Affiliation: | Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University,Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University,Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University,Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University,Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University and Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of CHemistry and Chemical Engineering, Southwest University |
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| Abstract: | |
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| Keywords: | HPLC Toona sinensis ultrasonic extraction Flavonoids organic acid content determinatio |
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