| 三种红豆杉BAPT基因的克隆及序列分析 |
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| 引用本文： | 韩丽,胡鸢雷,祝建波,林忠平.三种红豆杉BAPT基因的克隆及序列分析[J].石河子大学学报,2008,26(5). |
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| 作者姓名： | 韩丽 胡鸢雷 祝建波 林忠平 |
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| 作者单位： | 石河子大学生命科学学院农业生物技术实验室，北京大学生命科学学院 |
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| 摘 要： | 利用热启动PCR法分别从矮紫杉、欧洲红豆杉以及中国红豆杉基因组DNA中首次克隆到长度为1456bp的BAPT基因全长序列。序列分析结果表明:三种红豆杉BAPT基因序列的同源性达到了97.4%;将三种红豆杉的BAPT序列与NCBI上登录的BAPTcDNA序列相比对,发现其均含有1个核苷酸序列高度保守的110bp左右的内含子。
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| 关 键 词： | 红豆杉 BAPT基因 内含子 基因组 |
Cloning and Analysis of BAPT Gene from Three Different Taxus |
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| HAN Li,HU Yuan-lei,ZHU Jian-bo,LIN Zhong-ping.Cloning and Analysis of BAPT Gene from Three Different Taxus[J].Journal of Shihezi University(Natural Science),2008,26(5). |
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| Authors: | HAN Li HU Yuan-lei ZHU Jian-bo LIN Zhong-ping |
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| Institution: | HAN Li1,2,HU Yuan-lei2,ZHU Jian-bo1,LIN Zhong-ping1,2 |
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| Abstract: | In this study,Hot Start PCR was used to clone BAPT gene from Taxus cuspidata,Taxus.baccata and Taxus.chinensis.A length of 1456 bp PCR product was acquired.Sequence analysis showed that,the homology of BAPT genes from the three different taxus is 97.4%.Meanwhile,through the sequence alignment between the cDNA sequence of gene BAPT in NCBI and the three different taxus BAPT gene,we found that there is an intron about 110bp which is in a high sequence homology. |
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| Keywords: | taxus BAPT gene intron genomic DNA |
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