Large-scale purification of hepatitis B surface antigen using affinity chromatography |
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| Authors: | A R Neurath A M Prince J Giacalone |
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| Institution: | (1) The Lindsley F. Kimball Research Institute of The New York Blood Center, 10021 New York, New York, USA |
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| Abstract: | Summary Large-scale purification of hepatitis B surface antigen, applicable to the preparation of potential vaccines for prevention of hepatitis B, is described. The method involves the following steps: precipitation of serum with polyethylene glycol 6000, affinity chromatography on concanavalin A-Sepharose and on  -aminononyl-Sepharose, and isopycnic centrifugation.This study was supported by grant 09011 from the National Heart, Lung and Blood Institute. We thank Dr. B. Hollinger for testing HBsAg samples by a complement fixation test. |
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