| 滇西二高温温泉中Thermus高温菌的16S rRNA |
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| 引用本文： | 郭春雷,王涛,祝伟,柴丽红,张东华,崔晓龙,徐丽华,彭谦.滇西二高温温泉中Thermus高温菌的16S rRNA[J].云南大学学报(自然科学版),2003,25(5):458-462. |
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| 作者姓名： | 郭春雷 王涛 祝伟 柴丽红 张东华 崔晓龙 徐丽华 彭谦 |
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| 作者单位： | 教育部微生物资源开放研究重点实验室,云南大学,云南省微生物研究所,云南,昆明,650091 |
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| 基金项目： | 国家自然科学基金资助项目(3996003)，云南省应用基础基金资助项目(98C095M). |
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| 摘 要： | 通过对滇西二温泉纯培养和免培养法获得的Thermus菌株序列进行16SrDNA系统发育及16SrRNA二级结构比较,发现二温泉中至少存在Thermus属的2个潜在新种,它们在系统发育树上形成1个独立的地理簇群分支,它们的16SrRNAhelix10二级结构也与其它地区的Thermus属菌株不同,形成明显的地域进化特征.
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| 关 键 词： | 滇西 Thermus 16SrRNA 二级结构 |
| 文章编号： | 0258-7971(2003)05-0458-05 |
| 修稿时间： | 2002年12月12 |
The 16S rRNA of Thermus thermophiles from two high temperature hot springs of West Yunnan |
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| GUO Chun-lei,WANG Tao,ZHU Wei,CHAI Li-hong,ZHANG Dong-hua,CUI Xiao-long,XU Li-hua,PENG Qian.The 16S rRNA of Thermus thermophiles from two high temperature hot springs of West Yunnan[J].Journal of Yunnan University(Natural Sciences),2003,25(5):458-462. |
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| Authors: | GUO Chun-lei WANG Tao ZHU Wei CHAI Li-hong ZHANG Dong-hua CUI Xiao-long XU Li-hua PENG Qian |
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| Institution: | Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China |
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| Abstract: | Based on culture dependent method and culture independent method,several Thermus 16S rDNA sequences were amplified.The results of 16S rDNA phylogenetic analysis and 16S rRNA secondary stucture comparison showed that there are at least two novel Thermus species in the two hot springs and they constituted an independent cluster on the phylogenetic tree.The secondary structure of 16S rRNA helix 10 with novel evolution characters also showed differences with other Thermus species. |
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| Keywords: | West Yunnan Thermus 16S rRNA secondary structure |
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